Elevated mycobacterium avium subsp. paratuberculosis (MAP) antibody titer in Japanese multiple sclerosis

Fumie Hayashi; Noriko Isobe; Davide Cossu; Kazumasa Yokoyama; Ayako Sakoda; Takuya Matsushita; Nobutaka Hattori; Jun-Ichi Kira

doi:10.1016/j.jneuroim.2021.577701

Elevated mycobacterium avium subsp. paratuberculosis (MAP) antibody titer in Japanese multiple sclerosis

J Neuroimmunol. 2021 Nov 15:360:577701. doi: 10.1016/j.jneuroim.2021.577701. Epub 2021 Aug 25.

Authors

Fumie Hayashi¹, Noriko Isobe¹, Davide Cossu², Kazumasa Yokoyama³, Ayako Sakoda⁴, Takuya Matsushita¹, Nobutaka Hattori³, Jun-Ichi Kira⁵

Affiliations

¹ Department of Neurology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
² Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan; Department of Biomedical Sciences, Division of Microbiology and Virology, University of Sassari, Italy.
³ Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan.
⁴ Department of Neurology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; Department of Neurology, Brain and Nerve Center, Fukuoka Central Hospital, International University of Health and Welfare, Fukuoka, Japan.
⁵ Department of Neurology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; Department of Neurology, Brain and Nerve Center, Fukuoka Central Hospital, International University of Health and Welfare, Fukuoka, Japan; Translational Neuroscience Center, Graduate School of Medicine, School of Pharmacy at Fukuoka, International University of Health and Welfare, Fukuoka, Japan. Electronic address: kira@neuro.med.kyushu-u.ac.jp.

PMID: 34507015
DOI: 10.1016/j.jneuroim.2021.577701

Abstract

To investigate whether antibody production against mycobacterium avium subsp. paratuberculosis (MAP) is related to clinical characteristics of multiple sclerosis (MS) and human leukocyte antigen (HLA) alleles, IgG antibody against three MAP peptides and two human peptides homologous to MAP were measured in sera from 103 MS patients and 50 healthy controls (HCs). MS patients had higher IgG levels against MAP2694_295-303 (MAP2694-IgG) than HCs, while the other antibodies were comparable. Multivariate analysis demonstrated that higher MAP2694-IgG titers were associated with higher EDSS scores, but not with HLA alleles or dairy product consumption. Immune response against MAP may worsen MS disability.

Keywords: Expanded disability status scale; Multiple sclerosis; Mycobacterium avium subsp. paratuberculosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Antibodies, Bacterial / blood*
Antibody Specificity
Antigens, Bacterial / immunology
Bacterial Proteins / chemistry
Bacterial Proteins / immunology
Dairy Products
Diet
Female
Genes, MHC Class II
HLA-DRB1 Chains / genetics
Humans
Immunodominant Epitopes / immunology
Immunoglobulin G / blood*
Immunoglobulin G / immunology
Interferon Regulatory Factors / immunology
Japan / epidemiology
Male
Membrane Proteins / chemistry
Membrane Proteins / immunology
Middle Aged
Molecular Mimicry
Multiple Sclerosis / cerebrospinal fluid
Multiple Sclerosis / epidemiology
Multiple Sclerosis / genetics
Multiple Sclerosis / immunology*
Mycobacterium avium subsp. paratuberculosis / immunology*
Myelin Basic Protein / immunology
Oligoclonal Bands / cerebrospinal fluid
Peptide Fragments / immunology
Receptors, Antigen, T-Cell, alpha-beta / immunology
Sequence Alignment
Sequence Homology, Amino Acid
Severity of Illness Index

Substances

Antibodies, Bacterial
Antigens, Bacterial
Bacterial Proteins
HLA-DRB1 Chains
IRF5 protein, human
Immunodominant Epitopes
Immunoglobulin G
Interferon Regulatory Factors
Membrane Proteins
Myelin Basic Protein
Oligoclonal Bands
Peptide Fragments
Receptors, Antigen, T-Cell, alpha-beta