Isolation and Expansion of Mesenchymal Stem Cells from Murine Adipose Tissue

Natalia V Andreeva; Alexandra A Dalina; Alexander V Belyavsky

doi:10.21769/BioProtoc.2516

Isolation and Expansion of Mesenchymal Stem Cells from Murine Adipose Tissue

Bio Protoc. 2017 Aug 20;7(16):e2516. doi: 10.21769/BioProtoc.2516.

Authors

Natalia V Andreeva¹, Alexandra A Dalina², Alexander V Belyavsky¹

Affiliations

¹ Laboratory of Stem and Progenitor Cell Biology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
² Laboratory of Cell Proliferation, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.

Abstract

Mesenchymal stem cells (MSCs) are currently intensively studied due to significant promise which they represent for successful implementations of future cell therapy clinical protocols. This in turn emphasizes importance of careful preclinical studies of MSC effects in various murine disease models. The appropriate cell preparations with reproducible biological properties are important to minimize variability of results of experimental cell therapies. We describe here a simple protocol for isolation of murine MSCs from adipose tissues and their reproducible multi-log expansion under hypoxia conditions.

Keywords: Adipose tissue; Expansion; Hypoxia; Mesenchymal stem cells; Oxidative stress.