Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded Under Neurosphere Culture

Methods Mol Biol. 2022:2389:87-94. doi: 10.1007/978-1-0716-1783-0_8.

Abstract

Neural stem cells (NSCs) transplantation enhances plasticity and restores functions in neurological diseases. Therapeutic benefits of NSCs are due to their ability to replace the lost neurons and glial cells and also secreting a wide array of free and membrane-bound bioactive molecules that can reduce the hostility of diseased microenvironment, resolve inflammation, and rescue damaged neural cells. Membrane-encircled spherical nanostructures that are collectively known as extracellular vesicles (EVs) contain mRNA, miRNA, lipids, and specific proteins that affect different biological processes in cells located nearby or at far distances. Using EVs as an alternative non-cell-based therapy has gained huge attention, and developing methods for large-scale production of EVs is of great clinical importance. Here, we describe an efficient method to yield significant quantity of EVs from human NSCs that are expanded under free floating neurosphere assay culture system. Using the neurosphere assay in bioreactors under GMP-compliant conditions can result in scalable NSC-EVs required for human trials.

Keywords: Extracellular vesicles; Neural stem cells; Neurosphere assay; Production and isolation; Regenerative medicine.

MeSH terms

  • Biological Transport
  • Cell Separation
  • Cells, Cultured
  • Extracellular Vesicles* / metabolism
  • Humans
  • MicroRNAs / metabolism
  • Neural Stem Cells* / metabolism
  • Neurons / metabolism

Substances

  • MicroRNAs