Green and Efficient Extraction Approach for Polyphenol Recovery from Lotus Seedpods (Receptaculum Nelumbinis): Gas-Assisted Combined with Glycerol

Nina Bao; Marwan M A Rashed; Bianling Jiang; Kefeng Zhai; Zisheng Luo

doi:10.1021/acsomega.1c04190

Green and Efficient Extraction Approach for Polyphenol Recovery from Lotus Seedpods (Receptaculum Nelumbinis): Gas-Assisted Combined with Glycerol

ACS Omega. 2021 Sep 29;6(40):26722-26731. doi: 10.1021/acsomega.1c04190. eCollection 2021 Oct 12.

Authors

Nina Bao^{1

2}, Marwan M A Rashed¹, Bianling Jiang¹, Kefeng Zhai¹, Zisheng Luo^{2

3

4}

Affiliations

¹ Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biological and Food Engineering, Suzhou University, Suzhou 234000 Anhui, China.
² College of Biosystems Engineering and Food Science, Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou 310058 Zhejiang, China.
³ Ningbo Research Institute, Zhejiang University, Ningbo 315000 Zhejiang, China.
⁴ Fuli Institute of Food Science, Hangzhou 310058 Zhejiang, China.

Abstract

In this paper, the gas-assisted combined with glycerol extraction (GAGE) for polyphenol recovery from lotus seedpods (LSPs) was modeled and optimized. Box-Behnken design was applied to optimize the total polyphenol content (TPC) of LSP along with enhancing antioxidant activities using response surface methodology based on the TPC extraction yield (%), which was affected by glycerol concentration, time, temperature, and glycerol-to-solid ratio. The optimal conditions for the LSP extract were glycerol-to-solid ratio, 42 mL/g; time, 50 min; concentration of glycerol, 45%; and temperature, 70 °C. Ultra-high-pressure liquid chromatography integrated with triple-time-of-flight mass spectrophotometry (UPLC-Triple-TOF/MS) analysis revealed nine biologically active polyphenols. Furthermore, Fourier-transform infrared spectroscopy and scanning electron microscopy results demonstrated the effect and influence during extraction. The findings suggested that GAGE is a potential, green, and high-efficiency alternative that could be used to recover polyphenols from plant source byproducts.