Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities

Dalia M Dhingra; Aik T Ooi; David W Ruff

doi:10.1007/978-1-0716-1771-7_17

Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities

Methods Mol Biol. 2022:2386:289-307. doi: 10.1007/978-1-0716-1771-7_17.

Authors

Dalia M Dhingra¹, Aik T Ooi², David W Ruff²

Affiliations

¹ Mission Bio, Inc., South San Francisco, CA, USA. dhingra@missionbio.com.
² Mission Bio, Inc., South San Francisco, CA, USA.

PMID: 34766277
DOI: 10.1007/978-1-0716-1771-7_17

Abstract

Understanding the genomic landscape of cancer in single cells can be valuable for the characterization of molecular events that drive evolution of tumorigenesis and fostering progress in identifying druggable regimens for patient treatment scenarios. We report a new approach to measure multiple modalities simultaneously from up to 10,000 individual cells using microfluidics paired with next-generation sequencing. Our procedure determines targeted protein levels, mRNA transcript levels, and somatic gDNA sequence variations including copy number variants. This approach can resolve over 20 proteins, 100s of targeted transcripts, and DNA amplicons.

Keywords: Genomic DNA; Genotype; Multi-omics; Multimodal analysis; Phenotype; Protein expression; RNA expression; Single cell; Triomics.

MeSH terms

DNA / genetics
DNA Copy Number Variations
Genotype
High-Throughput Nucleotide Sequencing
Humans
Microfluidics*
Phenotype
RNA
Sequence Analysis, DNA
Workflow

Substances

RNA
DNA