Cyclodextrin metal-organic framework as vaccine adjuvants enhances immune responses

Drug Deliv. 2021 Dec;28(1):2594-2602. doi: 10.1080/10717544.2021.2012306.

Abstract

It is urgently needed to develop novel adjuvants for improving the safety and efficacy of vaccines. Metal-organic frameworks (MOFs), with high surface area, play an important role in drug delivery. With perfect biocompatibility and green preparation process, the γ-cyclodextrin metal-organic framework (γ-CD-MOF) fabricated with cyclodextrin and potassium suitable for antigen delivery. In this study, we modified γ-CD-MOF with span-85 to fabricate the SP-γ-CD-MOF as animal vaccine adjuvants. The ovalbumin (OVA) as the model antigen was encapsulated into particles to investigate the immune response. SP-γ-CD-MOF displayed excellent biocompatibility in vitro and in vivo. After immunization, SP-γ-CD-MOF loaded with OVA could induce high antigen-specific IgG titers and cytokine secretion. Meanwhile, SP-γ-CD-MOF also significantly improved the proliferation of spleen cells and activated and matured the bone marrow dendritic cells (BMDCs). The study showed the potential of SP-γ-CD-MOF in vaccine adjuvants and provided a novel idea for the development of vaccine adjuvants.

Keywords: immune responses; vaccine adjuvants; γ-cyclodextrin metal-organic framework.

MeSH terms

  • Adjuvants, Vaccine / administration & dosage
  • Adjuvants, Vaccine / pharmacology*
  • Animals
  • Animals, Outbred Strains
  • Bone Marrow Cells / drug effects
  • Cell Survival / drug effects
  • Chemistry, Pharmaceutical
  • Cytokines / drug effects
  • Female
  • Hemolysis / drug effects
  • Immunoglobulin G / drug effects
  • Metal-Organic Frameworks / chemistry*
  • Mice
  • Ovalbumin / administration & dosage
  • Ovalbumin / pharmacology*
  • RAW 264.7 Cells
  • Random Allocation
  • Spleen / drug effects
  • gamma-Cyclodextrins / chemistry*

Substances

  • Adjuvants, Vaccine
  • Cytokines
  • Immunoglobulin G
  • Metal-Organic Frameworks
  • gamma-Cyclodextrins
  • Ovalbumin
  • gamma-cyclodextrin

Supplementary concepts

  • Kunming mice

Grants and funding

This work was financially supported by National Science Foundation of China [21908183], Science and Technology Innovation and Entrepreneurship Seedling Key Projects of Sichuan Province [22MZGC0294] and Innovative Research Projects for Postgraduate Students, Southwest Minzu University [CX2020SZ50].