Extraction and quantification of metal-containing nanoparticles in marine shellfish based on single particle inductively coupled plasma-mass spectrometry technique

Yuan Sun; Yi Yang; Fei-Yun Tou; Zuo-Shun Niu; Xing-Pan Guo; Chang Liu; Jia Yan; Jia-Yuan Wu; Miao Xu; Li-Jun Hou; Min Liu

doi:10.1016/j.jhazmat.2021.127383

Extraction and quantification of metal-containing nanoparticles in marine shellfish based on single particle inductively coupled plasma-mass spectrometry technique

J Hazard Mater. 2022 Feb 15;424(Pt A):127383. doi: 10.1016/j.jhazmat.2021.127383. Epub 2021 Sep 30.

Authors

Yuan Sun¹, Yi Yang², Fei-Yun Tou¹, Zuo-Shun Niu¹, Xing-Pan Guo³, Chang Liu¹, Jia Yan¹, Jia-Yuan Wu¹, Miao Xu¹, Li-Jun Hou⁴, Min Liu³

Affiliations

¹ Key Laboratory of Geographic Information Science of the Ministry of Education, School of Geographical Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
² Key Laboratory of Geographic Information Science of the Ministry of Education, School of Geographical Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China; State Key Laboratory of Estuarine and Coastal Research, Yangtze Delta Estuarine Wetland Ecosystem Observation and Research Station, Ministry of Education & Shanghai, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China; Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, East China, Normal University, 500 Dongchuan Road, Shanghai 200241, China. Electronic address: yyang@geo.ecnu.edu.cn.
³ Key Laboratory of Geographic Information Science of the Ministry of Education, School of Geographical Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China; Institute of Eco-Chongming, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
⁴ State Key Laboratory of Estuarine and Coastal Research, Yangtze Delta Estuarine Wetland Ecosystem Observation and Research Station, Ministry of Education & Shanghai, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China.

PMID: 34879574
DOI: 10.1016/j.jhazmat.2021.127383

Abstract

Quantitative characterization of nanoparticles (NPs) in marine shellfish is critical to understanding the risks of bio-accumulation. Based on single particle (sp)ICP-MS and electron microscopy, a standardized protocol was developed to extract Ag, Au, and indigenous Ti-containing NPs from mussels. The optimal parameters are: dry sample extraction with tetramethylammonium hydroxide (TMAH), 5% (v/v) final concentration of TMAH, extraction at 25 ℃ for 12 h, and separation by centrifugation (3000 rpm for 5 min). The particle number recoveries of spiked Ag and Au NPs were 88 ± 0.9% and 95 ± 1.1%, respectively, while Ti-containing NPs had a particle number concentration of 8.2 × 10⁶ particles/mg and an average size of 70 nm in tested mussels. Furthermore, titanium oxide NPs, including rutile, anatase, and Magnéli phases (Ti_xO_2x-1) were found ubiquitously in 10 shellfish based on the optimal method. The particle number concentrations and average sizes of the Ti-containing NPs were 2.1 × 10⁶-8.4 × 10⁶ particles/mg and 70-80 nm, respectively. These Ti-containing NPs, such as TiO₂, accounted for about half of the Ti mass in shellfish, indicating that marine shellfish may be a significant sink for Ti-containing NPs.

Keywords: Method optimization; Mussels; Nanoparticles; SpICP-MS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bivalvia*
Mass Spectrometry
Metal Nanoparticles*
Nanoparticles*
Particle Size
Shellfish