A CASE toolkit for easy and efficient multiplex transgene-free gene editing

Plant Physiol. 2022 Mar 28;188(4):1843-1847. doi: 10.1093/plphys/kiab573.

Jin-Lei Liu^{1

2}, Meng-Meng Chen^{1

2}, Wen-Qiang Chen^{1

2}, Chun-Ming Liu^{1

2

3}, Yubing He^{4

5

6}, Xiu-Fen Song^{1

2}

¹ Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
² College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
³ School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China.
⁴ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
⁵ Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry, Nanjing Agricultural University, Nanjing 210095, China.
⁶ Excellence and Innovation Center, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.

Abstract

An integrated transgene-free multiplex gene-editing toolkit based on the Transgene Killer CRISPR technology greatly saves labor, time, and cost.