Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Tara R Hawkinson; Ramon C Sun

doi:10.1007/978-1-0716-2030-4_15

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Methods Mol Biol. 2022:2437:215-228. doi: 10.1007/978-1-0716-2030-4_15.

Authors

Tara R Hawkinson¹, Ramon C Sun^{2

3}

Affiliations

¹ Department of Neuroscience, University of Kentucky College of Medicine, Lexington, KY, USA.
² Department of Neuroscience, University of Kentucky College of Medicine, Lexington, KY, USA. Ramon.Sun@uky.edu.
³ Markey Cancer Center, Lexington, KY, USA. Ramon.Sun@uky.edu.

PMID: 34902151
DOI: 10.1007/978-1-0716-2030-4_15

Abstract

Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

Keywords: Alzheimer’s disease; FFPE tissue; Glycogen; Glycogen chain length; Isoamylase; MALDI-MSI.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Diagnostic Imaging
Glycogen
Lasers
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*

Substances

Glycogen

Abstract

Publication types

MeSH terms

Substances

Grants and funding