Isolation and in vitro cultivation of adrenal cells from mice

Linda Friedrich; Maria Schuster; Maria F Rubin de Celis; Ilona Berger; Stefan R Bornstein; Charlotte Steenblock

doi:10.1016/j.xpro.2021.100999

Isolation and in vitro cultivation of adrenal cells from mice

STAR Protoc. 2021 Dec 8;2(4):100999. doi: 10.1016/j.xpro.2021.100999. eCollection 2021 Dec 17.

Authors

Linda Friedrich¹, Maria Schuster¹, Maria F Rubin de Celis², Ilona Berger¹, Stefan R Bornstein^{1

3}, Charlotte Steenblock¹

Affiliations

¹ Department of Internal Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
² Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA.
³ Diabetes and Nutritional Sciences Division, King's College London, London WC2R 2LS, UK.

Abstract

The adrenal gland consists of two tissues, cortex and medulla, united under one capsule. Adrenal stem/progenitor cells play a key role in development and homeostasis. Here, we describe a protocol for generating primary cultures of adrenal cells from mice. We describe techniques for separating the cortex and medulla, generating spheroid cultures containing stem- and progenitor cells, and for the differentiation into steroidogenic and chromaffin cells, respectively. This protocol enables analysis of various treatments before, during, or after differentiation. For complete details on the use and execution of this protocol, please refer to Rubin de Celis et al. (2015), Steenblock et al. (2018), and Werdermann et al. (2021).

Keywords: Cell Biology; Cell Differentiation; Cell culture; Cell isolation; Microscopy; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adrenal Glands / cytology*
Animals
Cell Culture Techniques / methods*
Cell Differentiation / physiology
Cell Proliferation / physiology
Cell Separation / methods*
Cells, Cultured
Chromaffin Cells / cytology
Female
Male
Mice
Spheroids, Cellular / cytology
Stem Cells / cytology