Flipper Probes for the Community

Lea Assies; José García-Calvo; Francesca Piazzolla; Samantha Sanchez; Takehiro Kato; Luc Reymond; Antoine Goujon; Adai Colom; Javier López-Andarias; Karolína Straková; Dora Mahecic; Vincent Mercier; Margot Riggi; Noemi Jiménez-Rojo; Chloé Roffay; Giuseppe Licari; Maria Tsemperouli; Frederik Neuhaus; Alexandre Fürstenberg; Eric Vauthey; Sascha Hoogendoorn; Marcos Gonzalez-Gaitan; Andreas Zumbuehl; Kaori Sugihara; Jean Gruenberg; Howard Riezman; Robbie Loewith; Suliana Manley; Aurelien Roux; Nicolas Winssinger; Naomi Sakai; Stefan Pitsch; Stefan Matile

doi:10.2533/chimia.2021.1004

Flipper Probes for the Community

Chimia (Aarau). 2021 Dec 22;75(12):1004-1011. doi: 10.2533/chimia.2021.1004.

Authors

Lea Assies¹, José García-Calvo¹, Francesca Piazzolla¹, Samantha Sanchez¹, Takehiro Kato¹, Luc Reymond², Antoine Goujon¹, Adai Colom³, Javier López-Andarias¹, Karolína Straková¹, Dora Mahecic⁴, Vincent Mercier³, Margot Riggi⁵, Noemi Jiménez-Rojo³, Chloé Roffay³, Giuseppe Licari⁶, Maria Tsemperouli⁷, Frederik Neuhaus⁷, Alexandre Fürstenberg⁸, Eric Vauthey⁶, Sascha Hoogendoorn¹, Marcos Gonzalez-Gaitan³, Andreas Zumbuehl⁷, Kaori Sugihara⁹, Jean Gruenberg³, Howard Riezman³, Robbie Loewith¹⁰, Suliana Manley⁴, Aurelien Roux³, Nicolas Winssinger¹, Naomi Sakai¹, Stefan Pitsch¹¹, Stefan Matile¹²

Affiliations

¹ National Centre of Competence in Research (NCCR) Chemical Biology, 30 Quai Ernest-Ansermet, CH-1211 Geneva, Switzerland; Department of Organic Chemistry, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211 CH-Geneva, Switzerland.
² National Centre of Competence in Research (NCCR) Chemical Biology, 30 Quai Ernest-Ansermet, CH-1211 Geneva, Switzerland; Spirochrome AG, Chalberwiesenstrasse 4, CH-8260 Stein am Rhein, Switzerland.
³ National Centre of Competence in Research (NCCR) Chemical Biology, 30 Quai Ernest-Ansermet, CH-1211 Geneva, Switzerland; Department of Biochemistry, University of Geneva.
⁴ National Centre of Competence in Research (NCCR) Chemical Biology, 30 Quai Ernest-Ansermet, CH-1211 Geneva, Switzerland; École Polytechnique Fédérale de Lausanne - EPFL, SB Cubotron 427, CH-1015 Lausanne, Switzerland.
⁵ National Centre of Competence in Research (NCCR) Chemical Biology, 30 Quai Ernest-Ansermet, CH-1211 Geneva, Switzerland; Department of Biochemistry, University of Geneva; Department of Molecular Biology, University of Geneva.
⁶ Department of Physical Chemistry, University of Geneva.
⁷ National Centre of Competence in Research (NCCR) Chemical Biology, 30 Quai Ernest-Ansermet, CH-1211 Geneva, Switzerland; Department of Chemistry, University of Fribourg, 9 Chemin du Musée, CH-1700 Fribourg, Switzerland.
⁸ Department of Physical Chemistry, University of Geneva; Department of Inorganic and Analytical Chemistry, University of Geneva.
⁹ National Centre of Competence in Research (NCCR) Chemical Biology, 30 Quai Ernest-Ansermet, CH-1211 Geneva, Switzerland; Department of Physical Chemistry, University of Geneva.
¹⁰ National Centre of Competence in Research (NCCR) Chemical Biology, 30 Quai Ernest-Ansermet, CH-1211 Geneva, Switzerland; Department of Molecular Biology, University of Geneva.
¹¹ Spirochrome AG, Chalberwiesenstrasse 4, CH-8260 Stein am Rhein, Switzerland.
¹² National Centre of Competence in Research (NCCR) Chemical Biology, 30 Quai Ernest-Ansermet, CH-1211 Geneva, Switzerland; Department of Organic Chemistry, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211 CH-Geneva, Switzerland;, Email: stefan.matile@unige.ch.

PMID: 34920768
DOI: 10.2533/chimia.2021.1004

Abstract

This article describes four fluorescent membrane tension probes that have been designed, synthesized, evaluated, commercialized and applied to current biology challenges in the context of the NCCR Chemical Biology. Their names are Flipper-TR^®, ER Flipper-TR^®, Lyso Flipper-TR^®, and Mito Flipper-TR^®. They are available from Spirochrome.

MeSH terms

Coloring Agents
Fluorescent Dyes*
Membrane Potential, Mitochondrial*
Microscopy, Fluorescence

Substances

Coloring Agents
Fluorescent Dyes