Protocol for assessing senescence-associated lung pathologies in mice

STAR Protoc. 2021 Dec 4;2(4):100993. doi: 10.1016/j.xpro.2021.100993. eCollection 2021 Dec 17.

Abstract

Cellular senescence underlies tissue aging and aging-associated pathologies, as well as lung pathology. We and others have shown that elimination of senescent cells alleviates pulmonary diseases such as fibrosis and emphysema in animal models. We herein describe a protocol for assessing senescence-dependent lung phenotypes in mice. This protocol describes the use of ARF-DTR mice for semi-genetic elimination of lung senescent cells, followed by a pulmonary function test and the combination with pulmonary disease models to study lung pathologies. For complete details on the use and execution of this protocol, please refer to Hashimoto et al. (2016), Kawaguchi et al. (2021), and Mikawa et al. (2018).

Keywords: Cell Biology; Health Sciences; Model Organisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cellular Senescence*
  • Disease Models, Animal*
  • Female
  • Luminescent Measurements
  • Lung Diseases* / diagnostic imaging
  • Lung Diseases* / pathology
  • Lung* / cytology
  • Lung* / diagnostic imaging
  • Lung* / pathology
  • Male
  • Mice
  • Optical Imaging
  • Respiratory Function Tests