Reducing expression of TaOTUB1s decreases tiller number in wheat

Deng Ji Jiang; Xiao Hua Hao; Jing Zhang; Heng Tang; Fang Wang

doi:10.1080/15592324.2021.2018217

Reducing expression of TaOTUB1s decreases tiller number in wheat

Plant Signal Behav. 2021 Dec 2;16(12):2018217. doi: 10.1080/15592324.2021.2018217. Epub 2021 Dec 30.

Authors

Deng Ji Jiang¹, Xiao Hua Hao¹, Jing Zhang¹, Heng Tang², Fang Wang¹

Affiliations

¹ State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, China.
² College of Plant Protection, Shandong Agricultural University, Taian, Shandong, China.

Abstract

Tiller number is an important agronomic trait that affects crop yield. The roles of OTUB1 in regulating tiller numbers in rice have been reported. However, the roles of OTUB1 in wheat remain elusive. In this study, TaOTUB1s were identified in wheat. TaOTUB1 proteins were localized in the nucleus and cytoplasm. Compared with wild-type Fielder, TaOTUB1-RNAi transgenic wheat plants had fewer tillers. Similar to OTUB1 in rice, the yeast double hybrid indicated that the TaOTUB1-A protein could interact with TaSPL17 and TaUBC13 proteins. The results of quantitative real-time polymerase chain reaction revealed that the expression levels of TaOTUB1s decreased while those of TaSPL17 significantly improved in TaOTUB1-RNAi lines. These findings suggested that TaOTUB1s influenced tiller number in wheat.

Keywords: Otubain; TaOTUB1s; tiller number; wheat.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Oryza* / genetics
Phenotype
Plants, Genetically Modified / genetics
Triticum* / metabolism

Grants and funding

This research was supported by grants from the Major Program of the National Natural Science Foundation of China [grant number 919353024], the Natural Science Foundation of China [grant number 31971812] and the Shandong Natural Science Foundation [grant number ZR2019ZD15, ZR201911090314].