A dataset describing glycolytic inhibitors overcoming the underestimation of maximal mitochondrial oxygen consumption rate in oligomycin-treated cells

Juliana S Ruas; Edilene S Siqueira-Santos; Claudia D C Navarro; Roger F Castilho

doi:10.1016/j.dib.2021.107739

A dataset describing glycolytic inhibitors overcoming the underestimation of maximal mitochondrial oxygen consumption rate in oligomycin-treated cells

Data Brief. 2021 Dec 22:40:107739. doi: 10.1016/j.dib.2021.107739. eCollection 2022 Feb.

Authors

Juliana S Ruas¹, Edilene S Siqueira-Santos¹, Claudia D C Navarro¹, Roger F Castilho¹

Affiliation

¹ Department of Pathology, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP 13083-887, Brazil.

Abstract

Determination of oxygen consumption is one of the most valuable methodologies to evaluate mitochondrial (dys)function. Previous studies demonstrated that a widely used protocol, consisting of adding the ATP synthase inhibitor oligomycin before mitochondrial respiratory uncoupling by sequential addition of a protonophore (e.g., carbonyl cyanide 3-chlorophenyl hydrazone [CCCP]), may lead to underestimation of maximal oxygen consumption rate (OCR_max) and spare respiratory capacity (SRC) parameters in highly glycolytic tumor cell lines. In this dataset, we report the effects of the glycolytic inhibitors 2-deoxy-D-glucose, iodoacetic acid, and lonidamine on overcoming the underestimation of OCR_max and SRC in oligomycin-treated cells. We propose a protocol in which 2-deoxy-D-glucose is added after oligomycin and just before the sequential addition of CCCP to avoid underestimation of OCR_max and SRC parameters in A549, C2C12, and T98G cells. The oxygen consumption rates were determined in intact suspended cell lines using a high-resolution oxygraph device. The data can be used in several fields of research that require characterization of mitochondrial respiratory parameters in intact cells.

Keywords: 2-Deoxi-D-glucose; Mitochondria; Mitochondrial membrane potential; Oligomycin; Oxygen consumption; Spare respiratory capacity; Tumor cell.