The protocols presented here describe steps for cryosectioning tissue samples to be used in light microscopy methodologies including histochemistry, enzyme immunohistochemistry, and immunofluorescence. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Cryosectioning.
Keywords: cryopreservation; cryosectioning and immunofluorescence; tissue perfusion-fixation.
© 2022 Wiley Periodicals LLC.