CD36 regulates LPS-induced acute lung injury by promoting macrophages M1 polarization

Cell Immunol. 2022 Feb:372:104475. doi: 10.1016/j.cellimm.2021.104475. Epub 2022 Jan 11.

Abstract

M1 polarization of macrophages works as a promoter in pathogenesis of acute lung injury / acute respiratory distress syndrome (ALI/ARDS) by the secretion of pro-inflammatory cytokines and recruiting other inflammatory cells. Lipopolysaccharide (LPS), a critical component of the wall of gram-negative bacteria, can induce M1 polarization and ALI. Recently, cluster of differentiation 36 (CD36) has been reported to be associated with inflammatory responses. However, it has not yet been clarified whether CD36 in macrophages is involved in LPS-induced ALI. Herein, we demonstrated that in macrophages, LPS-induced ALI was regulated by CD36. Loss of CD36 attenuated LPS-induced ALI by reducing M1 polarization. Mechanistically, CD36 promoted macrophage M1 polarization by regulating CD14 associated with TLR4 during LPS stimulation. The findings of this study, clarified the mechanism of LPS-induced ALI through CD36 in macrophages, which provides a potential target for the prevention and treatment of ALI.

Keywords: Acute lung injury; CD36; Macrophage polarization; NF-κB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Lung Injury / etiology
  • Acute Lung Injury / immunology*
  • Acute Lung Injury / pathology
  • Adoptive Transfer
  • Animals
  • CD36 Antigens / antagonists & inhibitors
  • CD36 Antigens / genetics
  • CD36 Antigens / immunology*
  • Disease Models, Animal
  • Gene Knockout Techniques
  • Lipopolysaccharide Receptors / metabolism
  • Lipopolysaccharides / toxicity
  • Macrophages, Alveolar / classification*
  • Macrophages, Alveolar / drug effects
  • Macrophages, Alveolar / immunology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B / metabolism
  • Proto-Oncogene Proteins c-akt / metabolism
  • RAW 264.7 Cells
  • Signal Transduction
  • Toll-Like Receptor 4 / metabolism

Substances

  • CD36 Antigens
  • Cd14 protein, mouse
  • Cd36 protein, mouse
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • NF-kappa B
  • Tlr4 protein, mouse
  • Toll-Like Receptor 4
  • Proto-Oncogene Proteins c-akt