Monitoring NAD(H) and NADP(H) dynamics during organismal development with genetically encoded fluorescent biosensors

Ting Li; Yejun Zou; Shuning Liu; Yi Yang; Zhuo Zhang; Yuzheng Zhao

doi:10.1186/s13619-021-00105-4

Monitoring NAD(H) and NADP(H) dynamics during organismal development with genetically encoded fluorescent biosensors

Cell Regen. 2022 Feb 1;11(1):5. doi: 10.1186/s13619-021-00105-4.

Authors

Ting Li^#^{1

2}, Yejun Zou^#^{1

2}, Shuning Liu^{1

2}, Yi Yang¹, Zhuo Zhang^{3

4

5}, Yuzheng Zhao^{6

7

8}

Affiliations

¹ Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
² Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
³ Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China. zhangzhuo@ecust.edu.cn.
⁴ Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China. zhangzhuo@ecust.edu.cn.
⁵ Research Unit of New Techniques for Live-cell Metabolic Imaging, Chinese Academy of Medical Sciences, Beijing, China. zhangzhuo@ecust.edu.cn.
⁶ Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China. yuzhengzhao@ecust.edu.cn.
⁷ Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China. yuzhengzhao@ecust.edu.cn.
⁸ Research Unit of New Techniques for Live-cell Metabolic Imaging, Chinese Academy of Medical Sciences, Beijing, China. yuzhengzhao@ecust.edu.cn.

^# Contributed equally.

Abstract

Cell metabolism plays vital roles in organismal development, but it has been much less studied than transcriptional and epigenetic control of developmental programs. The difficulty might be largely attributed to the lack of in situ metabolite assays. Genetically encoded fluorescent sensors are powerful tools for noninvasive metabolic monitoring in living cells and in vivo by highly spatiotemporal visualization. Among all living organisms, the NAD(H) and NADP(H) pools are essential for maintaining redox homeostasis and for modulating cellular metabolism. Here, we introduce NAD(H) and NADP(H) biosensors, present example assays in developing organisms, and describe promising prospects for how sensors contribute to developmental biology research.

Keywords: Cell metabolism; Genetically encoded fluorescent sensors; NAD(H) and NADP(H); Organismal development; Real-time monitoring.

Publication types

Review