Achieving partial denitrification using organic matter in brewery wastewater as carbon source

Abstract

To find a cost-effective carbon source for partial denitrification (PD), brewery wastewater was utilized to test the viability of initiating PD. The S_bre (sludge from the biological treatment tank of Tsingtao Brewery Plant sewage treatment station) and S_lab (sludge from laboratory) were fed with brewery wastewater at COD_Cr/NO₃^--N (C/N) ratios of 8.0-10.0 and 5.0 for 95 days at 25 ± 1 °C, respectively. The mean NO₃^--N to NO₂^--N transformation ratio (NTR) in long-term operation was 40.0% in the S_bre system and 83.2% in the S_lab system. Batch tests with C/N ratio of 2.2-4.4 were conducted after 95 days incubation and the result suggested that C/N ratio of 4.3 ± 0.1 contributed more to NO₂^--N accumulation in both systems. Thauera bacteria, known to be beneficial for NO₂^--N accumulation, became the dominant community. The relative abundances of Thauera on day 95 in the S_bre and S_lab system were 83.36% and 79.11%, respectively.

Keywords: Electron acceptor; Microbial community; NO(2)(−)-N accumulation; NO(3)(−)-N to NO(2)(−)-N transformation ratio (NTR); Partial denitrification (PD).