Hydrodynamic Injection for Developing NASH Model

Haichuan Wang; Xin Chen

doi:10.1007/978-1-0716-2128-8_3

Hydrodynamic Injection for Developing NASH Model

Methods Mol Biol. 2022:2455:31-39. doi: 10.1007/978-1-0716-2128-8_3.

Authors

Haichuan Wang¹, Xin Chen²

Affiliations

¹ Department of Bioengineering and Therapeutic Sciences and Liver Center, University of California, San Francisco, CA, USA.
² Department of Bioengineering and Therapeutic Sciences and Liver Center, University of California, San Francisco, CA, USA. xin.chen@ucsf.edu.

Abstract

The hydrodynamic tail vein injection (HTVi) is a technique that is used to deliver plasmid genes into live mice or rats. The HTVi leads to the in vivo transfection of exogenous DNA primarily in the liver, serving as a reliable approach of establishing animal models for the study of liver diseases. The nonalcoholic steatohepatitis (NASH) is liver inflammation and damage resulting from an accumulation of fat in the liver. With the rising prevalence of obesity worldwide, NASH is becoming an increasingly common health problem. The pathogenesis of NASH is a multi-step process involving complicated pathways. The molecular mechanisms of NASH remain poorly understood. Here, we describe the use of HTVi to establish animal models for the study of NASH.

Keywords: Hydrodynamic tail vein injection; Mouse; Nonalcoholic steatohepatitis; Rat.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Disease Models, Animal
Hydrodynamics
Liver / metabolism
Liver Cirrhosis / metabolism
Mice
Mice, Inbred C57BL
Non-alcoholic Fatty Liver Disease* / etiology
Non-alcoholic Fatty Liver Disease* / metabolism
Obesity / metabolism
Rats

Abstract

Publication types

MeSH terms

Grants and funding