Saccharides from Arctium lappa L. root reduce platelet activation and thrombus formation in a laser injury thrombosis mouse model

Yongjuan Ruan; Yanzhong Ding; Xiaowei Li; Chunyang Zhang; Mengyu Wang; Mengduan Liu; Lu Wang; Junhui Xing; Liang Hu; Xiaoyan Zhao; Zhongren Ding; Jianzeng Dong; Yangyang Liu

doi:10.3892/etm.2022.11274

Saccharides from Arctium lappa L. root reduce platelet activation and thrombus formation in a laser injury thrombosis mouse model

Exp Ther Med. 2022 May;23(5):344. doi: 10.3892/etm.2022.11274. Epub 2022 Mar 22.

Authors

Yongjuan Ruan¹, Yanzhong Ding¹, Xiaowei Li^{1

2}, Chunyang Zhang^{2

3}, Mengyu Wang¹, Mengduan Liu¹, Lu Wang¹, Junhui Xing¹, Liang Hu¹, Xiaoyan Zhao¹, Zhongren Ding^{1

4}, Jianzeng Dong^{1

5}, Yangyang Liu^{1

2}

Affiliations

¹ Department of Cardiology, Cardiovascular Center, Henan Key Laboratory of Hereditary Cardiovascular Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
² Department of General Thoracic Surgery, Hami Central Hospital, Hami, Xinjiang 839000, P.R. China.
³ Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
⁴ School of Pharmacy, Tianjin Medical University, Tianjin 300203, P.R. China.
⁵ National Clinical Research Centre for Cardiovascular Diseases, Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China.

Abstract

Arctium lappa L., also known as burdock, is a popular medicinal plant in traditional Chinese medicine due to its potential therapeutic properties. Saccharides from Arctium lappa L. root (ALR-S) have been extensively studied for their anti-inflammatory and anti-diabetes effects. Platelets play a pivotal role in thrombosis. The present study describes the effects of ALR-S on platelet activation and thrombosis using a laser injury thrombosis in vivo model. The study also measured the effects of ALR-S on platelet activation by analysing aggregation, ATP release, platelet spreading, adhesion and clot retraction in vitro. Specifically, the effects were ALR-S concentration-dependent inhibition of platelet aggregation and ATP release. Activated platelets pretreated with ALR-S showed diminished CD62P expression levels and fibrinogen binding, as measured by flow cytometry. ALR-S inhibited platelet spreading on fibrinogen and adhesion on collagen under shear. ALR-S attenuated platelet activation by decreasing oxidative stress and thrombus formation. These results demonstrated the antiplatelet effects of ALR-S, suggesting the antithrombotic and cardiovascular protective activities of ALR-S as a functional food.

Keywords: Arctium lappa L; laser injury thrombosis model; platelet aggregation; saccharides; thrombosis.

Grants and funding

Funding: This study was financially supported by the National Natural Science Foundation of China (grant no. 81903603), the China Postdoctoral Science Foundation (grant no. 2020M6722920, the Henan Province Medical Science and Technology Key Project (Union construction) (grant no. 2018020067), the Natural Science Foundation of Henan Province (Youth Project) (grant no. 202300410396) and the Young Talent Promotion Project from Henan Province (grant no. 2021HYTP043).