A xanthene-based fluorescent probe for detection of peroxynitrite in living cells and zebrafish

Chenlu Liang; Wei Shu; Rubing Han; Hao Kang; Xiaoli Zhang; Jing Jing; Rubo Zhang; Xiaoling Zhang

doi:10.1016/j.saa.2022.121264

A xanthene-based fluorescent probe for detection of peroxynitrite in living cells and zebrafish

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Sep 5:277:121264. doi: 10.1016/j.saa.2022.121264. Epub 2022 Apr 13.

Authors

Chenlu Liang¹, Wei Shu², Rubing Han¹, Hao Kang¹, Xiaoli Zhang¹, Jing Jing³, Rubo Zhang⁴, Xiaoling Zhang⁵

Affiliations

¹ Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electro Photonic Conversion Materials, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China.
² School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255000, PR China. Electronic address: jdshuwei@163.com.
³ Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electro Photonic Conversion Materials, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China; School of Medical Technology, Beijing Institute of Technology, Beijing 100081, PR China.
⁴ Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electro Photonic Conversion Materials, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: zhangrubo@bit.edu.cn.
⁵ Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electro Photonic Conversion Materials, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China; School of Medical Technology, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: zhangxl@bit.edu.cn.

PMID: 35439675
DOI: 10.1016/j.saa.2022.121264

Abstract

Peroxynitrite (ONOO^-) is one of quite critical reactive oxygen species that acts critical roles in a number of diverse biological functions and pathological events. Notably, excessive ONOO^- will lead to sorts of diseases. Thus, monitoring of endogenous ONOO^- levels will be conducive to exploring the physiological activities and functions of ONOO^-. Here, a simple turn-on fluorescent probe named DMX is reported using CN bond as the ONOO^- recognition site and xanthene as the fluorophore. DMX possessed a good linear dependence with ONOO^- concentration (0-9 μM), highly sensitive detection (DL = 37 nM), and excellent selectivity towards ONOO^-. What is more, the biological experiments reveal that DMX is able to be utilized to track exogenous/endogenous ONOO^- employing confocal laser scanning microscopy. Visualization of ONOO^- in zebrafish was also successfully conducted, suggesting that DMX might be used to study ONOO^- roles in vivo. We believe that DMX will have potential for exploring the pivotal role of ONOO^- during all sorts of physiological and pathological activities.

Keywords: Bioimaging; CN bond; Fluorescence probe; ONOO(−).

MeSH terms

Animals
Fluorescent Dyes* / chemistry
Microscopy, Confocal
Peroxynitrous Acid*
Xanthenes
Zebrafish

Substances

Fluorescent Dyes
Xanthenes
Peroxynitrous Acid