A method to visualize cell motility in fluorescence-labeled mouse-ear dermal explants is described. This approach allows cell and matrix dynamics to be visualized in physiologically relevant, three-dimensional (3D) environments. This Basic Protocol for the preparation of mouse-ear dermal explants can be optimized and applied to any tissue explant and cell type. © 2022 Wiley Periodicals LLC.
Keywords: collagen; dermis; extracellular matrix; live-cell imaging; motility; three-dimensional.
© 2022 Wiley Periodicals LLC.