Protein Purification and Digestion Methods for Bacterial Proteomic Analyses

Abstract

Reproducible protein extraction is critical for the quantitative analysis of bacterial proteomes. While a wide range of techniques exist, there is no one-size-fits-all solution that will be suitable for all applications. In this report, we describe a set of standard extraction methods that have been adapted for a range of bacterial proteome analyses.

Keywords: Mass spectrometry; Proteome purification; Quantitative proteomics; Sample preparation; Surfactant.