Determination of testosterone in serum by magnetic molecularly imprinted polymer-coupled nano-ESI-MS

Mingying Ma; Tianhao Wu; Gongwei Sun; Sichun Zhang

doi:10.1016/j.ab.2022.114719

Determination of testosterone in serum by magnetic molecularly imprinted polymer-coupled nano-ESI-MS

Anal Biochem. 2022 Sep 15:653:114719. doi: 10.1016/j.ab.2022.114719. Epub 2022 May 23.

Authors

Mingying Ma¹, Tianhao Wu¹, Gongwei Sun², Sichun Zhang³

Affiliations

¹ Department of Chemistry, Tsinghua University, Beijing, 100084, China.
² Department of Chemistry, Tsinghua University, Beijing, 100084, China. Electronic address: bjtasi@163.com.
³ Department of Chemistry, Tsinghua University, Beijing, 100084, China. Electronic address: sczhang@mail.tsinghua.edu.cn.

PMID: 35618034
DOI: 10.1016/j.ab.2022.114719

Abstract

Monitoring clinical biomarkers, such as testosterone in serum, is important for disease assessment. Due to the very low concentration of testosterone in serum, we have developed a new strategy for its enrichment in serum samples by magnetic molecularly imprinted polymers (MMIPs) technology and detection by nano-electrospray ionization mass spectrometry (Nano-ESI-MS). Testosterone was selectively extracted and enriched by the imprinted polymers on the surface of magnetic particles and the complex matrix was eliminated from the serum. The linear calibration curve was in the range of 0.1-10 μg/L and the limit of detection was 11.4 ng/L. The recovery and repeatability of the spiked serum were satisfactory. These results demonstrate that the proposed method is a promising approach for quantitative analysis of testosterone in serum.

Keywords: Magnetic molecularly imprinted polymers (MMIPs); Nano-ESI-MS; Quantitative; Testosterone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Magnetic Phenomena
Magnetics
Molecular Imprinting* / methods
Molecularly Imprinted Polymers
Testosterone

Substances

Molecularly Imprinted Polymers
Testosterone