Effective Biotransformation of Variety of Guaiacyl Lignin Monomers Into Vanillin by Bacillus pumilus

Kangjia Zuo; Huanan Li; Jianhui Chen; Qiuping Ran; Mengtian Huang; Xinxin Cui; Lili He; Jiashu Liu; Zhengbing Jiang

doi:10.3389/fmicb.2022.901690

Effective Biotransformation of Variety of Guaiacyl Lignin Monomers Into Vanillin by Bacillus pumilus

Front Microbiol. 2022 May 11:13:901690. doi: 10.3389/fmicb.2022.901690. eCollection 2022.

Authors

Kangjia Zuo^{1

2}, Huanan Li^{1

2}, Jianhui Chen^{1

2}, Qiuping Ran^{1

2}, Mengtian Huang^{1

2}, Xinxin Cui^{1

2}, Lili He^{1

2}, Jiashu Liu^{1

2}, Zhengbing Jiang^{1

2}

Affiliations

¹ State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, China.
² Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, China.

Abstract

Biotransformation has gained increasing attention due to its being an eco-friendly way for the production of value-added chemicals. The present study aimed to assess the potential of Bacillus pumilus ZB1 on guaiacyl lignin monomers biotransformation for the production of vanillin. Consequently, isoeugenol, eugenol, and vanillyl alcohol could be transformed into vanillin by B. pumilus ZB1. Based on the structural alteration of masson pine and the increase of total phenol content in the supernatant, B. pumilus ZB1 exhibited potential in lignin depolymerization and valorization using masson pine as the substrate. As the precursors of vanillin, 61.1% of isoeugenol and eugenol in pyrolyzed bio-oil derived from masson pine could be transformed into vanillin by B. pumilus ZB1. Four monooxygenases with high specific activity were identified that were involved in the transformation process. Thus, B. pumilus ZB1 could emerge as a candidate in the biosynthesis of vanillin by using wide guaiacyl precursors as the substrates.

Keywords: Bacillus pumilus; bio-oil; biotransformation; monooxygenase; vanillin.