Identification of collagen 1α3 in teleost fish species and typical collision induced internal fragmentations

Anne J Kleinnijenhuis; Frédérique L van Holthoon; Bastiaan van der Steen

doi:10.1016/j.fochx.2022.100333

Identification of collagen 1α3 in teleost fish species and typical collision induced internal fragmentations

Food Chem X. 2022 May 17:14:100333. doi: 10.1016/j.fochx.2022.100333. eCollection 2022 Jun 30.

Authors

Anne J Kleinnijenhuis¹, Frédérique L van Holthoon¹, Bastiaan van der Steen²

Affiliations

¹ Triskelion, Reactorweg 47A, 3542 AD Utrecht, The Netherlands.
² Rousselot Expertise Center, Meulestedekaai 81, B-9000 Ghent, Belgium.

Abstract

In contrast to collagens 1α1 and 1α2, the more obscure collagen 1α3 is sparsely mentioned in literature. In skin collagen type 1 of teleosts (bony fish), however, the chain occurs in a heterotrimer together with collagens 1α1 and 1α2, which makes it one of the most abundant proteins in teleosts. As teleost fish species and gelatin (hydrolysate) prepared from their skin are a major source for food products and nutraceuticals, the goal of the study was to selectively identify collagen 1α3 in several fish species. Fish skin extracts and fish skin gelatins were analyzed using LC-MS. Depending on the amount of available genetic information different approaches were used to identify collagen 1α3. Additionally, collagen-specific collision induced internal fragmentations are discussed, which are important to consider during data analysis. Ultimately the presence of collagen 1α3 could be confirmed using LC-MS in multiple fish species.

Keywords: Collagen 1α3; Fish; Gelatin; Internal fragmentation; LC-MS; Teleost.