In silico profiling of analgesic and antihyperglycemic effects of ethanolic leaves extract of Amischotolype mollissima: Evidence from in vivo studies

Maisha Maliha Medha; Hiron Saraj Devnath; Biswajit Biswas; Bishwajit Bokshi; Samir Kumar Sadhu

doi:10.1016/j.sjbs.2022.103312

In silico profiling of analgesic and antihyperglycemic effects of ethanolic leaves extract of Amischotolype mollissima: Evidence from in vivo studies

Saudi J Biol Sci. 2022 Jul;29(7):103312. doi: 10.1016/j.sjbs.2022.103312. Epub 2022 May 20.

Authors

Maisha Maliha Medha¹, Hiron Saraj Devnath¹, Biswajit Biswas², Bishwajit Bokshi¹, Samir Kumar Sadhu¹

Affiliations

¹ Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.
² Department of Pharmacy, Jashore University of Science & Technology, Jashore 7408, Bangladesh.

Abstract

The aim of this study is to assess the antioxidative profile and related pharmacological potentialities of the ethanolic extract of Amischotolype mollissima leaves, traditionally used in treating pain, injury, malarial fever, epilepsy and hyperacidity, followed by a computational approach for the analysis of bioactive compounds identified by GC-MS. In GC-MS analysis, the extract yielded ten compounds, with 4,6-di-t-butyl-2-alpha-methyl benzyl phenol having the highest amount. In vitro investigation of the antioxidative properties of the plant was conducted with 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical and hydrogen peroxide scavenging assays. The amounts of secondary metabolites phenolics, flavonoids, and tannins were measured at 142 mg GAE/g, 534 mg QE/g, and 110 mg GAE/g, respectively. An acute toxicity study was carried out on mice, which revealed no toxicity up to the dosage of 4000 mg/kg bw. For the dosages of extract at 250 and 500 mg/kg bw, the writhing response test induced by acetic acid exhibited a statistically significant (p < 0.05) analgesic effect in mice. The oral glucose tolerance test (OGTT) and alpha-glucosidase enzyme inhibitory activity assay were used to examine the antihyperglycemic potential, in which the extract reduced the blood glucose level to 6.22 mmol/l and 3.82 mmol/l, at dosages of 250 and 500 mg/kg bw, respectively at 60 min in OGTT even though no activity was observed in the α-glucosidase enzyme inhibitory assay. In an antibacterial assay, the extract's minimum inhibitory concentration (MIC) against E. coli, P. aeruginosa, and S. aureus was determined to be 8, 16, and 8 µg/ml, respectively. This study shows that the usage of A. mollissima leaves in folklore medication is justified.

Keywords: ANOVA, one-way analysis of variance; Amischotolype mollissima; Analgesic; Antihyperglycemic; Antioxidative; Commelinacea; GC-MS analysis; GC–MS, gas chromatography-mass spectroscopy; In silico profiling; NIST, National Institute of Standards and Technology; PDB ID, protein data bank identification code; SC50, scavenging concentration 50%; SD, standard deviation; UV, ultra-violet; bw, body weight.