High-Throughput Analysis to Identify Activators of Notch Signaling

Rachael Guenter; Jacob Eide; Herbert Chen; J Bart Rose; Renata Jaskula-Sztul

doi:10.1007/978-1-0716-2201-8_5

High-Throughput Analysis to Identify Activators of Notch Signaling

Methods Mol Biol. 2022:2472:49-56. doi: 10.1007/978-1-0716-2201-8_5.

Authors

Rachael Guenter¹, Jacob Eide², Herbert Chen¹, J Bart Rose¹, Renata Jaskula-Sztul³

Affiliations

¹ Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
² Department of Otolaryngology - Head and Neck Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
³ Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA. rjsztul@uabmc.edu.

PMID: 35674891
DOI: 10.1007/978-1-0716-2201-8_5

Abstract

The Notch pathway regulates many cellular functions in a context-dependent manner. Depending on the cell type, either the activation or inhibition of Notch signaling can influence many processes such as cellular proliferation, specification, differentiation, and survival. The activation of Notch signaling has been shown to have therapeutic advantages in some cancers, thus having a method to identify Notch-activating compounds is needed. In this chapter we outline a method for high-throughput analysis of potential Notch pathway activators in a pancreatic neuroendocrine tumor cell line as an example. We also include the steps for subsequent validation of results and preclinical testing.

Keywords: Cancer therapeutics; High-throughput analysis; Luciferase reporter plasmid; Notch activation; Notch pathway; Notch signaling.

MeSH terms

Cell Proliferation
Humans
Neoplasms*
Receptors, Notch* / metabolism
Signal Transduction

Substances

Receptors, Notch