Long Non-Coding RNA AP000695.2 Acts as a Novel Prognostic Biomarker and Regulates the Cell Growth and Migration of Lung Adenocarcinoma

Chunyan Wang; Jishu Guo; Rongyan Jiang; Chenyang Wang; Chenglong Pan; Zhi Nie; Xiulin Jiang

doi:10.3389/fmolb.2022.895927

Long Non-Coding RNA AP000695.2 Acts as a Novel Prognostic Biomarker and Regulates the Cell Growth and Migration of Lung Adenocarcinoma

Front Mol Biosci. 2022 May 24:9:895927. doi: 10.3389/fmolb.2022.895927. eCollection 2022.

Authors

Chunyan Wang¹, Jishu Guo², Rongyan Jiang³, Chenyang Wang¹, Chenglong Pan¹, Zhi Nie^{4

5}, Xiulin Jiang⁶

Affiliations

¹ Department of Pathology, First Affiliated Hospital of Kunming Medical University, Kunming, China.
² School of Ecology and Environmental Science, Yunnan University, Kunming, China.
³ Department of Cardiovascular Medicine, the Bozhou Hospital Affiliated to Anhui Medical University, Bozhou Anhui, China.
⁴ Department of Neurology, First Affiliated Hospital of Kunming Medical University, Kunming, China.
⁵ Yunnan Province Clinical Research Center for Neurological Diseases, Kunming, China.
⁶ Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing, China.

Abstract

Long non-coding RNAs (lncRNAs) are tumor-associated biological molecules and have been found to be implicated in the progression of lung adenocarcinoma (LUAD). LncRNA-AP000695.2 (ENSG00000248538) is a long non-coding RNA (lncRNA) that is widely increased in many tumor types including lung adenocarcinoma (LUAD). However, the aberrant expression profile, clinical significance, and biological function of AP000695.2 in human lung adenocarcinoma (LUAD) need to be further investigated. This study mines key prognostic AP000695.2 and elucidates its potential role and molecular mechanism in regulating the proliferation and metastasis of LUAD. Here, we discovered that AP000695.2 was significantly upregulated in lung adenocarcinoma tissues compared with healthy adjacent lung tissue and higher in LUAD cell lines than in normal human bronchial epithelial cell lines. A higher expression of AP000695.2 was positively correlated with aggressive clinicopathological characteristics, and AP000695.2 served as an independent prognostic indicator for the overall survival, disease-free survival, and progression-free survival in patients with LUAD. Receiver operating curve (ROC) analysis revealed the significant diagnostic ability of AP000695.2 (AUC = 0.838). Our in vivo data confirmed that AP000695.2 promotes the proliferation, migration, and invasion of LUAD cells. GSEA results suggested that AP000695.2 co-expressed genes were mainly enriched in immune-related biological processes such as JAK-STAT signaling pathway and toll-like receptor signaling pathway. Single-sample GSEA analysis showed that AP000695.2 is correlated with tumor-infiltrating immune cells in lung adenocarcinoma. Our findings confirmed that AP000695.2 was involved in the progression of lung adenocarcinoma, providing a novel prognostic indicator and promising diagnostic biomarker in the future.

Keywords: cell apoptosis; cell growth; cell migration; cell proliferation; lncRNA; lung adenocarcinoma; prognosis biomarker.