Alternative c-MYC mRNA Transcripts as an Additional Tool for c-Myc2 and c-MycS Production in BL60 Tumors

Biomolecules. 2022 Jun 16;12(6):836. doi: 10.3390/biom12060836.

Abstract

While studying c-Myc protein expression in several Burkitt lymphoma cell lines and in lymph nodes from a mouse model bearing a translocated c-MYC gene from the human BL line IARC-BL60, we surprisingly discovered a complex electrophoretic profile. Indeed, the BL60 cell line carrying the t(8;22) c-MYC translocation exhibits a simple pattern, with a single c-Myc2 isoform. Analysis of the c-MYC transcripts expressed by tumor lymph nodes in the mouse λc-MYC (Avy/a) showed for the first time five transcripts that are associated with t(8;22) c-MYC translocation. The five transcripts were correlated with the production of c-Myc2 and c-MycS, and loss of c-Myc1. The contribution of these transcripts to the oncogenic activation of the t(8;22) c-MYC is discussed.

Keywords: BL60 cell line; c-Myc protein; lymph nodes; lymphoma cell lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Burkitt Lymphoma* / genetics
  • Genes, myc*
  • Mice
  • RNA, Messenger / genetics
  • Transcription, Genetic
  • Translocation, Genetic

Substances

  • RNA, Messenger

Grants and funding

This study was supported by grants from the Institut National du Cancer (INCa), the Ligue Contre le Cancer (Equipe Labellisée Ligue), the Comité Orientation Recherche Cancer (CORC), the Limousin Region and the Haute Vienne and Corrèze committees of the Ligue Nationale contre le Cancer and by the Lyons Club de Corrèze. We do not have a number available.