Branched poly(ethylenimine) carbon dots-MnO2 nanosheets based fluorescent sensory system for sensing of malachite green in fish samples

Xiaowei Mu; Xin Liu; Xiwen Ye; Wei Zhang; Lu Li; Pinyi Ma; Daqian Song

doi:10.1016/j.foodchem.2022.133517

Branched poly(ethylenimine) carbon dots-MnO₂ nanosheets based fluorescent sensory system for sensing of malachite green in fish samples

Food Chem. 2022 Nov 15:394:133517. doi: 10.1016/j.foodchem.2022.133517. Epub 2022 Jun 18.

Authors

Xiaowei Mu¹, Xin Liu¹, Xiwen Ye¹, Wei Zhang¹, Lu Li², Pinyi Ma³, Daqian Song⁴

Affiliations

¹ Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
² State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China. Electronic address: luli@jlu.edu.cn.
³ Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China. Electronic address: mapinyi@jlu.edu.cn.
⁴ Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China. Electronic address: songdq@jlu.edu.cn.

PMID: 35749877
DOI: 10.1016/j.foodchem.2022.133517

Abstract

Malachite green (MG) is an organic dye compound that is frequently used as a fungicide and antiseptic in aquaculture. However, human or animal exposure to MG causes carcinogenic, teratogenic and mutagenic effects. Herein, a novel fluorescent assay was designed for the detection of MG using manganese dioxide nanosheets (MnO₂ NS) as an energy acceptor to quench the fluorescence of branched poly(ethylenimine) carbon dots (BPEI-CDs) via Förster resonance energy transfer. When butyrylcholinesterase is introduced to form thiocholine in the presence of S-butyrylthiocholine iodide, MnO₂ NS can be recovered by thiocholine to Mn²⁺, resulting in restoration of the fluorescence of BPEI-CDs. Exploiting these changes in fluorescence intensity in the above system, a fluorescence probe was successfully developed for the quantitative detection of MG. Besides, this assay was applied to fish samples, verifying the high potential for practical application of the proposed sensor for the monitoring of MG in aquatic products.

Keywords: Butyrylcholinesterase; Carbon dots; Forster resonance energy transfer; Malachite green.

MeSH terms

Animals
Aquaculture
Butyrylcholinesterase
Carbon / chemistry
Fishes
Fluorescent Dyes / chemistry
Limit of Detection
Luminescent Measurements* / methods
Manganese Compounds / chemistry
Nanostructures / chemistry
Oxides
Polyethyleneimine
Quantum Dots* / chemistry
Rosaniline Dyes / analysis
Rosaniline Dyes / chemistry
Thiocholine

Substances

Fluorescent Dyes
Manganese Compounds
Oxides
Rosaniline Dyes
malachite green
Thiocholine
Carbon
Polyethyleneimine
Butyrylcholinesterase