In Situ Imaging of mRNA Splicing Variants by SpliceRCA

Xiaojun Ren; Jinghong Li

doi:10.1007/978-1-0716-2521-7_12

In Situ Imaging of mRNA Splicing Variants by SpliceRCA

Methods Mol Biol. 2022:2537:197-209. doi: 10.1007/978-1-0716-2521-7_12.

Authors

Xiaojun Ren^{1

2}, Jinghong Li³

Affiliations

¹ Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing, China.
² Department of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing, China.
³ Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing, China. jhli@mail.tsinghua.edu.cn.

PMID: 35895266
DOI: 10.1007/978-1-0716-2521-7_12

Abstract

We present a method for multiplexed in situ imaging of individual RNA splicing variants. This method enables quantifying RNA splicing variants with single-molecule resolution, discriminating splicing isoforms with single-base precision as well as analyzing the subcellular localization of transcripts. With this technology, it is possible to study cell heterogeneity of gene expression, potentially helping to decipher rich diversity in posttranscriptional function and assist clinical monitoring.

Keywords: Fluorescence imaging; In situ imaging; RNA splicing variants; Rolling circle amplification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alternative Splicing*
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA Splicing*
RNA, Messenger / genetics
RNA, Messenger / metabolism

Substances

Protein Isoforms
RNA, Messenger