Ultrasonic treatment of Dendrobium officinale polysaccharide enhances antioxidant and anti-inflammatory activity in a mouse D-galactose-induced aging model

Wenhui Chu; Pan Wang; Zhe Ma; Lin Peng; Zongmin Wang; Zilin Chen

doi:10.1002/fsn3.2867

Ultrasonic treatment of Dendrobium officinale polysaccharide enhances antioxidant and anti-inflammatory activity in a mouse D-galactose-induced aging model

Food Sci Nutr. 2022 Apr 1;10(8):2620-2630. doi: 10.1002/fsn3.2867. eCollection 2022 Aug.

Authors

Wenhui Chu¹, Pan Wang², Zhe Ma¹, Lin Peng¹, Zongmin Wang³, Zilin Chen²

Affiliations

¹ School of Life Science Taizhou University Taizhou China.
² Traditional Chinese Medicine Industry Development and Promotion Center of Pan'an County Pan'an China.
³ School of Agriculture and Food Engineering Shandong University of Technology Zibo China.

Abstract

Utilization of the biological macromolecule Dendrobium officinale polysaccharide (DOP) as a functional ingredient is limited by its high intrinsic viscosity and molecular weight. The goal of the present study was to improve rheological properties of DOP by ultrasonic treatment. Such a treatment resulted in the degradation of DOP and consequent reduction of rheological properties. Among DOP samples treated with ultrasonication at low (L), medium (M), and high (H) power intensities (25, 50, 75 w/cm²), M-DOP displayed the highest reactive oxygen species (ROS) and reactive nitrogen species (RNS) radical scavenging activity in vitro. In a mouse D-galactose (D-Gal)-induced aging model, M-DOP significantly increased activities of antioxidant enzymes and reduced levels of pro-inflammatory cytokines in liver. Real-time polymerase chain reaction (RT-PCR) analysis indicated that M-DOP upregulated messenger RNA (mRNA) expression of anti-inflammatory/antioxidant proteins such as Nrf2 (nuclear factor erythroid 2-related factor), hemeoxygenase-1 (HO-1), and NAD(P)H:quinone oxidoreductase (NQO1) in liver. In summary, M-DOP displayed a strong radical scavenging activity in vitro, and ameliorated liver injury in the mouse aging model through the promotion of Nrf2/HO-1/NQO1 signaling pathway.

Keywords: Dendrobium officinale; D‐galactose; antioxidant activity; anti‐inflammatory activity; polysaccharide.