Retromer subunit, CfVps35 is required for growth development and pathogenicity of Colletotrichum fructicola

Xi-Ya Li; Sheng-Pei Zhang; Li He

doi:10.1186/s12863-022-01084-4

Retromer subunit, CfVps35 is required for growth development and pathogenicity of Colletotrichum fructicola

BMC Genom Data. 2022 Aug 28;23(1):68. doi: 10.1186/s12863-022-01084-4.

Authors

Xi-Ya Li^{1

2}, Sheng-Pei Zhang^{1

3}, Li He^{4

5

6}

Affiliations

¹ Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Central South University of Forestry and Technology, Changsha, China.
² Key Laboratory for Non-wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Changsha, China.
³ Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha, China.
⁴ Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Central South University of Forestry and Technology, Changsha, China. T20061078@csuft.edu.cn.
⁵ Key Laboratory for Non-wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Changsha, China. T20061078@csuft.edu.cn.
⁶ Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha, China. T20061078@csuft.edu.cn.

Abstract

Background: Tea oil is widely used as edible oil in China, which extracted from the seeds of Camellia oleifera. In China, the national oil-tea camellia planting area reached 4.533 million hectares, the output of oil-tea camellia seed oil was 627 000 tons, and the total output value reached 18.3 billion dollars. Anthracnose is the common disease of Ca. oleifera, which affected the production and brought huge economic losses. Colletotrichum fructicola is the dominant pathogen causing anthracnose in Ca. oleifera. The retromer complex participates in the intracellular retrograde transport of cargos from the endosome to the trans-Golgi network in eukaryotes. Vacuolar protein sorting 35 is a core part of the retromer complex. This study aimed to investigate the role of CfVps35 in C. fructicola.

Results: The CfVPS35 gene was deleted, resulting in reduced mycelial growth, conidiation, and response to cell wall stresses. Further analysis revealed that CfVps35 was required for C. fructicola virulence on tea oil leaves. In addition, the ΔCfvps35 mutant was defective in glycogen metabolism and turgor during appressorium development.

Conclusion: This study illustrated that the crucial functions of CfVps35 in growth, development, and pathogenicity.

Keywords: Camellia oleifera; CfVps35; Colletotrichum fructicola; Pathogenicity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Camellia*
Colletotrichum*
Plant Diseases
Tea
Virulence

Substances

Tea

Supplementary concepts

Colletotrichum fructicola