A new technique for separating platelet-rich plasma by a copolymer device － without a centrifugation process

Lien-Hung Huang; Cheng-Shyuan Rau; Wen-Hong Zeng; Tsu-Hsiang Lu; Yi-Chan Wu; Yi-Hong Chiu; Kai-Ming Wu; Chia-Wen Tsai; Chia-Wei Lin; Chia-Jung Wu; Yi-Chang Chung; Ching-Hua Hsieh

doi:10.1016/j.biopha.2022.113481

A new technique for separating platelet-rich plasma by a copolymer device － without a centrifugation process

Biomed Pharmacother. 2022 Sep:153:113481. doi: 10.1016/j.biopha.2022.113481. Epub 2022 Aug 2.

Authors

Affiliations

¹ Department of Neurosurgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan.
² Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung University Rd., Nan-Tsu District, Kaohsiung 811, Taiwan.
³ Department of Plastic Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan.
⁴ BiomiMed Tech Ltd. Co., 6F-3., Kai-Hsuan Rd., East District, Tainan 701, Taiwan.
⁵ Research Center of Health and Biomimetic Technology, National University of Kaohsiung, Kaohsiung University Rd., Nan-Tsu District, Kaohsiung 811, Taiwan.
⁶ Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung University Rd., Nan-Tsu District, Kaohsiung 811, Taiwan; Research Center of Health and Biomimetic Technology, National University of Kaohsiung, Kaohsiung University Rd., Nan-Tsu District, Kaohsiung 811, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Da-Hsueh Rd.,Tainan 701, Taiwan. Electronic address: ycchung@nuk.edu.tw.
⁷ Department of Plastic Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan. Electronic address: m93chinghua@gmail.com.

PMID: 36076501
DOI: 10.1016/j.biopha.2022.113481

Abstract

Background: Platelet-rich plasma (PRP) is beneficial for clinical applications in various medical fields. Although commercial PRP preparation kits are already available in the market, most of these kits employ centrifugation.

Methods: We used a new cationic copolymer coating on a polyurethane (PU) sponge to promote platelet separation from the blood. This copolymer showed no cytotoxicity against cell viability or hemolysis. We further evaluated the efficiency of the new PRP preparation device by comparing it with that of a commercially available kit (RegenKit-THT).

Results: We demonstrated that PRP obtained using copolymer device contains high concentrations of platelets and angiogenic growth factors (epidermal growth factor, vascular endothelial growth factor-A, growth differentiation factor 2, and interleukin-8). The separated PRP also displayed beneficial effects on cell migration, angiogenesis, and matrix metalloproteinase gene expression.

Conclusion: Based on these results, we developed a cationic copolymer-coated PU sponge as a PRP preparation device without the need for any centrifugation.

Keywords: Angiogenesis; Centrifugation; Copolymer; Growth factors; Platelet-rich plasma.

MeSH terms

Blood Platelets
Centrifugation / methods
Intercellular Signaling Peptides and Proteins / metabolism
Platelet-Rich Plasma* / metabolism
Vascular Endothelial Growth Factor A* / metabolism

Substances

Intercellular Signaling Peptides and Proteins
Vascular Endothelial Growth Factor A