Pretreatment with millet-derived selenylated soluble dietary fiber ameliorates dextran sulfate sodium-induced colitis in mice by regulating inflammation and maintaining gut microbiota balance

Weihao Wang; Fang Kou; Juan Wang; Zhigang Quan; Shuting Zhao; Yifei Wang; Xin Hu; Hunan Sun; Longkui Cao

doi:10.3389/fnut.2022.928601

Pretreatment with millet-derived selenylated soluble dietary fiber ameliorates dextran sulfate sodium-induced colitis in mice by regulating inflammation and maintaining gut microbiota balance

Front Nutr. 2022 Sep 7:9:928601. doi: 10.3389/fnut.2022.928601. eCollection 2022.

Authors

Weihao Wang^{1

2}, Fang Kou^{1

3}, Juan Wang¹, Zhigang Quan¹, Shuting Zhao¹, Yifei Wang¹, Xin Hu¹, Hunan Sun^{1

4}, Longkui Cao^{1

2}

Affiliations

¹ College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, China.
² National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, China.
³ Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, South Korea.
⁴ College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, China.

Abstract

Inflammatory activation and intestinal flora imbalance play key roles in the development and progression of inflammatory bowel disease (IBD). Soluble dietary fiber (SDF) and selenium have been proven to be effective for preventing and relieving IBD. This study investigated and compared the therapeutic efficacy of millet-derived selenylated-soluble dietary fiber (Se-SDF) against dextran sulfate sodium (DSS)-induced colitis in mice alone or through the synergistic interaction between selenium and SDF. In female mice, Se-SDF markedly alleviated body weight loss, decreased colon length, reduced histological damage scores, and enhanced IL-10 expression to maintain the barrier function of intestinal mucosa compared to male mice. The 16S rRNA sequence analysis further indicated that pretreatment with Se-SDF restored the gut microbiota composition in female mice by increasing the relative abundance of Lactobacillus and the Firmicutes/Bacteroidetes ratio. In conclusion, these findings demonstrated that Se-SDF can protect against DSS-induced colitis in female mice by regulating inflammation and maintaining gut microbiota balance. This study, therefore, provides new insights into the development of Se-SDF as a supplement for the prevention and treatment of colitis.

Keywords: Se-SDF; colitis; gut microbiome; inflammatory response; millet.