Valorphins alter physicochemical characteristics of phosphatidylcholine membranes: Datasets on lipid packing, bending rigidity, specific electrical capacitance, dipole potential, vesicle size

Victoria Vitkova; Galya Staneva; Rusina Hazarosova; Stela I Georgieva; Iva Valkova; Krassimira Antonova; Petаr Todorov

doi:10.1016/j.dib.2022.108716

Valorphins alter physicochemical characteristics of phosphatidylcholine membranes: Datasets on lipid packing, bending rigidity, specific electrical capacitance, dipole potential, vesicle size

Data Brief. 2022 Nov 2:45:108716. doi: 10.1016/j.dib.2022.108716. eCollection 2022 Dec.

Authors

Victoria Vitkova¹, Galya Staneva², Rusina Hazarosova², Stela I Georgieva³, Iva Valkova⁴, Krassimira Antonova¹, Petаr Todorov⁵

Affiliations

¹ Institute of Solid State Physics, Bulgarian Academy of Sciences, 72 Tzarigradsko Chaussee Blvd., 1784 Sofia, Bulgaria.
² Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, Sofia 1113, Bulgaria.
³ Department of Analytical Chemistry, University of Chemical Technology and Metallurgy, 8 Kliment Ohridski Blvd., 1756 Sofia, Bulgaria.
⁴ Department of Chemistry, Faculty of Pharmacy, Medical University, 2 Dunav Str. 1000 Sofia, Bulgaria.
⁵ Department of Organic Chemistry, University of Chemical Technology and Metallurgy, 8 Kliment Ohridski Blvd., 1756 Sofia, Bulgaria.

Abstract

Endogenous hemorphins are being intensively investigated as therapeutic agents in neuropharmacology, and also as biomarkers in mood regulation, inflammation and oncology. The datasets collected herein report physicochemical parameters of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine membranes in the presence of VV-hemorphin-5 (Val-Val-Tyr-Pro-Trp-Thr-Gln) and analogues, modified at position 1 and 7 by the natural amino acid isoleucine or the non-proteinogenic 2-aminoisobutyric, 2,3-diaminopropanoic or 2,4-diaminobutanoic amino acids. These peptides have been previously screened for nociceptive activity and were chosen accordingly. The present article contains fluorescence spectroscopy data of Laurdan- and di-8-ANEPPS- labelled large unilamellar vesicles (LUV) providing the degree of hydration and dipole potential of lipid bilayers in the presence of VV-hemorphin-5 analogues. Lipid packing is accessible from Laurdan intensity profiles and generalized polarization datasets reported herein. The data presented on fluorescence intensity ratios of di-8-ANEPPS dye provide dipole potential values of phosphatidylcholine-valorphin membranes. Vesicle size and electrophoretic mobility datasets included refer to the effect of valorphins on the size distribution and $ζ$ -potential of POPC LUVs. Investigation of physicochemical properties of peptides such as diffusion coefficients and heterogeneous rate constant relates to elucidation of transport mechanisms in living cells. Voltammetric data of valorphins are presented together with square-wave voltammograms of investigated peptides for calculation of their heterogeneous electron transfer rate constants. Datasets from the thermal shape fluctuation analysis of quasispherical 'giant' unilamellar vesicles (GUV) are provided to quantify the influence of hemorphin incorporation on the membrane bending elasticity. Isothermal titration calorimetric data on the thermodynamics of peptide-lipid interactions and the binding affinity of valorphin analogues to phosphatidylcholine membranes are reported. Data of frequency-dependent deformation of GUVs in alternating electric field are included together with the values of the specific electrical capacitance of POPC-valorphin membranes. The datasets reported in this article can underlie the formulation and implementation of peptide-based strategies in pharmacology and biomedicine.

Keywords: Aib, 2-aminoisobutyric acid; CV, cyclic voltammetry; DPV, differential pulse voltammetry; Dab, 2,4-diaminobutanoic acid; Dap, 2,3-diaminopropanoic acid; Di-8-ANEPPS; Di-8-ANEPPS, 4-(2-[6-(Dioctylamino)-2-naphthalenyl]ethenyl)-1-(3-sulfopropyl)pyridinium inner salt; Fluctuation analysis; GP, generalized polarization; GUV, giant unilamellar vesicle; Gln, glutamine (Q); ITC, isothermal titration calorimetry; ITO, indium tin oxide; Ile, isoleucine (I); Isothermal titration calorimetry; LUV, large unilamellar vesicle; Laurdan; Lipid vesicles; PC, phosphatidylcholine; PDMS, polydimethylsiloxane; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; Pro, proline (P); Thr, threonine (T); Trp, tryptophan (W); Tyr, tyrosine (Y); VV-hemorphin-5, Val-Val-Tyr-Pro-Trp-Thr-Gln-NH2; Val, valine (V); Voltammetry; АTSF, analysis of thermal shape fluctuations.