Using Micropatterned Supported Lipid Bilayers to Probe the Mechanosensitivity of Signaling Receptors

Zhongwen Chen

doi:10.1007/978-1-0716-2851-5_18

Using Micropatterned Supported Lipid Bilayers to Probe the Mechanosensitivity of Signaling Receptors

Methods Mol Biol. 2023:2600:283-289. doi: 10.1007/978-1-0716-2851-5_18.

Author

Zhongwen Chen¹

Affiliation

¹ Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China. chenzw@sioc.ac.cn.

PMID: 36587104
DOI: 10.1007/978-1-0716-2851-5_18

Abstract

The ligand-receptor complexes in the cell-cell and cell-ECM interfaces mediate the mechanical coupling of cells to their microenvironment and transduce downstream signals to modulate cell functions. In this chapter I describe a microfabrication strategy to prepare a substrate of spatially segregated supported lipid bilayers and ECM components on which cells can form juxtacrine receptor signaling complexes and integrin adhesions simultaneously. This platform is specifically applicable for microscopically monitoring the signal transduction of each receptor, as well as the modulatory effects of receptor signaling on integrin adhesions and cell behaviors.

Keywords: Cell receptor; ECM; Integrin adhesion; Juxtacrine signaling; Microfabrication; Receptor tyrosine kinase; Supported lipid bilayer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Adhesion / physiology
Cell Membrane / metabolism
Integrins / metabolism
Lipid Bilayers* / chemistry
Signal Transduction* / physiology

Substances

Lipid Bilayers
Integrins