Mechanical and biological characteristics of 3D fabricated clay mineral and bioceramic composite scaffold for bone tissue applications

Abstract

3D printing technology provides a platform to fabricate a wide range of structures and complex geometry-based scaffolds through computer-aided design (CAD). This study investigates the possibility of developing Bentonite(BEN)/Hydroxyapatite(HAP) scaffold with different HAP wt% (25, 50, 75) using a 3D printing technique (Robocasting) for potential bone tissue applications. Thermal stability of the composites was characterized in TGA and rheological properties of slurries were observed to have different viscosity and shear stress, especially BEN-HAP 50 wt% achieves all criteria for high-quality printing. The fabricated scaffolds were subjected to sintering from 200 °C to 1000 °C for proper densification and attained a maximum compression strength of 52 MPa at 1000 °C for the printed structures. Changes in crystallinity and functional groups were observed as well with respective sintering temperatures. In this study, we also discussed the extrusion and rheological properties of the composite slurry. Porosity, water absorption, degradation and density were studied to understand the physical properties of the sintered scaffolds. The biological characteristics of the scaffold were studied using MG63 cell lines In vitro biocompatibility study and expressed 91% of viability for the 1000 °C sintered samples under controlled culture conditions.

Keywords: Bone tissue engineering; Clay mineral/ceramic scaffold; Cost-effective; Robocasting.