Immunogenicity analysis of the E. coli expressed structural protein VP1 of persistent infection foot-and-mouth disease virus

Huan Tang; Hailong Wang; Li Yang; Hong Chen; Lingbao Kong; Xiu Xin

doi:10.1016/j.virol.2023.01.004

Immunogenicity analysis of the E. coli expressed structural protein VP1 of persistent infection foot-and-mouth disease virus

Virology. 2023 Feb:579:111-118. doi: 10.1016/j.virol.2023.01.004. Epub 2023 Jan 6.

Authors

Huan Tang¹, Hailong Wang², Li Yang¹, Hong Chen¹, Lingbao Kong¹, Xiu Xin³

Affiliations

¹ Institute of Pathogenic Microbiology, College of Biological Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China; Nanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang, 330045, China.
² Center for Experimental Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, 330045, China.
³ Institute of Pathogenic Microbiology, College of Biological Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China; Nanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang, 330045, China. Electronic address: xinxiujiangxi@sina.com.

PMID: 36634474
DOI: 10.1016/j.virol.2023.01.004

Abstract

The persistent infection of FMDV in cloven hoofed animals has made the epidemic prevention and control more difficult. VP1 is the main immunogenic protein and first candidate of vaccine development for FMDV prevention. However, the mutation of VP1 in host cell with persistent infection FMDV (PI-FMDV) caused the change of its immunogenicity. Hence, it is imperative to establish the expression system for VP1 of PI-FMDV (PI-VP1) and re-evaluate its immunogenicity. In this study, the PI-VP1 with His-tag was cloned into pET-28a vector. PI-VP1 protein was expressed and purified in E. coli, and further the antiserum of immunized mice was analyzed. Results showed that purified PI-VP1 protein produced a good humoral and cellular immune response after immunizing mice. Furthermore, our study showed that the antiserum could not only neutralize PI-FMDV, but also prevent the adsorption of WT-FMDV. In summarize, our work provides valuable implications for the FMDV vaccines and therapeutics development.

Keywords: Immunogenicity; Persistent infection; Subunit vaccine; Type O foot-And-mouth disease virus; VP1 structural protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral
Capsid Proteins / chemistry
Escherichia coli / genetics
Foot-and-Mouth Disease Virus* / genetics
Foot-and-Mouth Disease*
Mice
Persistent Infection
Viral Vaccines*

Substances

Capsid Proteins
Antibodies, Viral
Viral Vaccines