Protocol for standardized intrathymic injection in mice

Tsung-Lin Tsai; Pei-Yuan Tsai; Ivan L Dzhagalov; Chia-Lin Hsu

doi:10.1016/j.xpro.2022.102010

Protocol for standardized intrathymic injection in mice

STAR Protoc. 2023 Mar 17;4(1):102010. doi: 10.1016/j.xpro.2022.102010. Epub 2023 Jan 12.

Authors

Tsung-Lin Tsai¹, Pei-Yuan Tsai², Ivan L Dzhagalov³, Chia-Lin Hsu⁴

Affiliations

¹ Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; Taiwan International Graduate Program in Molecular Medicine, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, Taiwan.
² Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei 112, Taiwan. Electronic address: kiya6918@gmail.com.
³ Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
⁴ Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; Taiwan International Graduate Program in Molecular Medicine, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, Taiwan. Electronic address: clhsu@nycu.edu.tw.

Abstract

Currently available intrathymic injection techniques cause postoperative complications or difficulties in equipment acquisition. Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2022).¹.

Keywords: Developmental Biology; Immunology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Injections*
Mice
Thymus Gland*