Identification of 2-Methylthio-methylenethio-N6 -(cis-4-hydroxyisopentenyl)adenosine (msms2 io6 A37 ) as a Novel Modification at Adenosine 37 of tRNAs from Salmonella typhimurium

Chembiochem. 2023 Mar 14;24(6):e202300019. doi: 10.1002/cbic.202300019. Epub 2023 Feb 20.

Abstract

Post-transcriptional modifications of tRNA nucleotide are important determinants in folding, structure and function. We have successfully identified and characterized a new modified base named 2-methylthio-methylenethio-N6 -(cis-4-hydroxyisopentenyl)adenosine, which is present at position 37 in some tRNAs. We also showed that this new modified adenosine is derived from the known 2-methylthio-methylenethio-N6 -(isopentenyl)adenosine nucleoside by a catalytic cycle of the tRNA-diiron monooxygenase, MiaE, present in Salmonella typhimurium.

Keywords: LC-MS; hydroxylation; tRNA modifications; tRNA-diiron monooxygenase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine*
  • Isopentenyladenosine / chemistry
  • Mixed Function Oxygenases / chemistry
  • RNA, Transfer / chemistry
  • Salmonella typhimurium* / genetics

Substances

  • Adenosine
  • RNA, Transfer
  • Isopentenyladenosine
  • Mixed Function Oxygenases