Quantitative proteomic analysis reveals the effects of mu opioid agonists on HT22 cells

Xutong Zhang; Yani Lou; Dongxu Zheng; Jialin Lu; Dansi Qi

doi:10.3389/fphar.2022.1022449

Quantitative proteomic analysis reveals the effects of mu opioid agonists on HT22 cells

Front Pharmacol. 2023 Jan 9:13:1022449. doi: 10.3389/fphar.2022.1022449. eCollection 2022.

Authors

Xutong Zhang¹, Yani Lou¹, Dongxu Zheng¹, Jialin Lu¹, Dansi Qi²

Affiliations

¹ Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
² Department of Pathology, Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

Abstract

Introduction: At present, the mu opioid receptor is the most important neuroaesthetics receptor in anesthesiology research, and the damage that it does to the nervous system is unknown. Methods: We investigated the effects of loperamide, an agonist of the mu opioid receptor, on protein expression in HT22 cells using stable isotope labeling of amino acids in cell culture (SILAC), immobilized metal affinity chromatography (IMAC) enrichment, and high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 7,823 proteins were identified. Results and Discussion: Bioinformatic analysis revealed that mu opioid receptor agonism can induce distinct changes in the proteome of HT22 cells. These findings improve our understanding of narcotic drugs.

Keywords: HT22 cell; LC-MS/MS; loperamide; mu opioid agonist; nerve injury; proteomic.

Grants and funding

This study was funded by the Wenzhou Science and Technology Bureau (Grant No. Y20180067).