Pexophagy in plants: a mechanism to remit cells from oxidative damage caused under high-intensity light

Shino Goto-Yamada; Kazusato Oikawa; Yasuko Hayashi; Shoji Mano; Kenji Yamada; Mikio Nishimura

doi:10.1080/15548627.2023.2175570

Pexophagy in plants: a mechanism to remit cells from oxidative damage caused under high-intensity light

Autophagy. 2023 May;19(5):1611-1613. doi: 10.1080/15548627.2023.2175570. Epub 2023 Feb 9.

Authors

Shino Goto-Yamada¹, Kazusato Oikawa², Yasuko Hayashi³, Shoji Mano^{4

5}, Kenji Yamada¹, Mikio Nishimura^{2

6}

Affiliations

¹ Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
² Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.
³ Department of Science, Faculty of Science, Niigata University, Niigata, Japan.
⁴ Laboratory of Organelle Regulation, National Institute for Basic Biology, Okazaki, Japan.
⁵ Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Japan.
⁶ Faculty of Science and Engineering, Konan University, Kobe, Japan.

Abstract

Light is essential for plant growth, but excessive light energy produces reactive oxygen species (ROS), which can seriously damage cells. Mutants defective in ATG (autophagy related) genes show light intensity-dependent leaf damage and ROS accumulation. We found that autophagy is one of the crucial systems in protecting plants from ROS-induced damage by removing oxidative peroxisomes. Damaged peroxisomes are targeted by the PtdIns3P marker and specifically engulfed by phagophores labeled by ATG18a-GFP. Under high-intensity light, huge peroxisome aggregates are induced and captured by vacuolar membranes. Research provides a deeper understanding of plant stress response to light irradiation.

Keywords: Autophagy; ROS; microautophagy; peroxisome; pexophagy; plant.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autophagy* / physiology
Macroautophagy*
Oxidative Stress
Peroxisomes / metabolism
Plants
Reactive Oxygen Species / metabolism

Substances

Reactive Oxygen Species

Grants and funding

This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas (no. 22120007 to M.N.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT); a Grants-in-Aid for Scientific Research (no. 17K07467 to Y.H. and K.O., no. 20370024 to M.N., and nos. 26440157 and 20570045 to S.M.) from Japan Society for the Promotion of Science (JSPS); a SONATA Grant (UMO-2019/35/D/NZ3/04500 to S.G.-Y.) from National Science Centre Poland; a TEAM Grant (TEAM/2017-4/41 to K.Y.) from the Foundation for Polish Science; and the Wyeth Foundation to M.N..