High-throughput phenotyping salt tolerance in JUNCAOs by combining prompt chlorophyll a fluorescence with hyperspectral spectroscopy

Haiyong Weng; Mingyang Wu; Xiaobin Li; Libin Wu; Jiayi Li; Tolulope Opeyemi Atoba; Jining Zhao; RenYe Wu; Dapeng Ye

doi:10.1016/j.plantsci.2023.111660

High-throughput phenotyping salt tolerance in JUNCAOs by combining prompt chlorophyll a fluorescence with hyperspectral spectroscopy

Plant Sci. 2023 May:330:111660. doi: 10.1016/j.plantsci.2023.111660. Epub 2023 Feb 21.

Authors

Haiyong Weng¹, Mingyang Wu¹, Xiaobin Li¹, Libin Wu¹, Jiayi Li¹, Tolulope Opeyemi Atoba¹, Jining Zhao¹, RenYe Wu², Dapeng Ye³

Affiliations

¹ College of Mechanical and Electrical Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Key Laboratory of Agricultural Information Sensoring Technology, College of Mechanical and Electrical Engineering, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
² College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: renye.wu@fafu.edu.cn.
³ College of Mechanical and Electrical Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Key Laboratory of Agricultural Information Sensoring Technology, College of Mechanical and Electrical Engineering, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: ydp@fafu.edu.cn.

PMID: 36822504
DOI: 10.1016/j.plantsci.2023.111660

Abstract

The planting of salt-tolerant plants is regarded as the one of important measurements to improve the saline-alkali lands. The outstanding biological properties of JUNCAOs have made them candidates to improve and utilize saline-alkali lands. At present, little attention has been paid to developing a non-destructive and high throughput approach to evaluate the salt tolerance of JUNCAO. To close the gaps, three typical JUNCAOs (A.donax. No.1, A.donax. No.5 and A.donax. No.10) were evaluated by combining prompt chlorophyll a fluorescence (ChlF) with hyperspectral spectroscopy (HS). The results showed that salt stress reduced relative stem growth, water content, and total chlorophyll content but enhanced the malondialdehyde (MDA) content. It caused a significant change in chlorophyll a fluorescence kinetics with an appearance of L-, K- and J-band, implying damaging energetic connectivity between PSII units, uncoupling of the oxygen evolving complex (OEC) and inhibition of the Q_A^-reoxidation. The negative impact of salt stress on JUNCAOs increased with the increasing level of salt concentration. Effect on spectral reflectance in the in the visible region with shifts on red edge position (REP) and blue edge position (BEP) to shorter wavelength was also found in salt stress plants. Combining principal component analysis (PCA) with the membership function method based on spectral indices and JIP-test parameters could well screen JUNCAOs salt tolerant ability with the highest for A.donax. NO.10 but lowest for A.donax. NO.1, which was the same as that of using conventional approach. The results demonstrate that prompt ChlF coupling with HS could provide potentials for non-invasively and high-throughput phenotyping salt tolerance in JUNCAOs.

Keywords: Hyperspectral spectroscopy; JUNCAO; Membership function; Prompt chlorophyll a fluorescence; Salt tolerance.

MeSH terms

Chlorophyll A
Chlorophyll* / analysis
Fluorescence
Salt Stress
Salt Tolerance*
Spectrum Analysis

Substances

Chlorophyll A
Chlorophyll