Dissecting LuxS/AI-2 quorum sensing system-mediated phenyllactic acid production mechanisms of Lactiplantibacillus plantarum L3

Yunmei Chai; Qingwen Ma; Xin Nong; Xinyue Mu; Aixiang Huang

doi:10.1016/j.foodres.2023.112582

Dissecting LuxS/AI-2 quorum sensing system-mediated phenyllactic acid production mechanisms of Lactiplantibacillus plantarum L3

Food Res Int. 2023 Apr:166:112582. doi: 10.1016/j.foodres.2023.112582. Epub 2023 Feb 10.

Authors

Yunmei Chai¹, Qingwen Ma², Xin Nong¹, Xinyue Mu¹, Aixiang Huang³

Affiliations

¹ Department of Food Science, College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
² Department of Food Science, College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, China; Yunnan Normal University, Kunming 650092, Yunnan, China.
³ Department of Food Science, College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, China. Electronic address: aixianghuang@126.com.

PMID: 36914344
DOI: 10.1016/j.foodres.2023.112582

Abstract

The phenyllactic acid (PLA) produced by lactic acid bacteria (LAB) inhibits fungi and facilitates the quality control of fermented milk. A strain of Lactiplantibacillus plantarum L3 (L. plantarum L3) with high PLA production was screened in the pre-laboratory, but the mechanism of its PLA formation is unclear. The amount of autoinducer-2 (AI-2) increased with increasing culture time, as did cell density and PLA. The results in this study suggest that PLA production in L. plantarum L3 may be regulated by the LuxS/AI-2 Quorum Sensing (QS) system. Tandem mass tag (TMT) quantitative proteomics analysis showed that a total of 1291 differentially expressed proteins (DEPs) were quantified in the incubated for 24 h compared with the incubated for 2 h, of which 516 DEPs were up-regulated and 775 DEPs were down-regulated. Among them, S-ribosomal homocysteine lyase (luxS), aminotransferase (araT), and lactate dehydrogenase (ldh) are the key proteins for PLA formation. The DEPs were mainly involved in the QS pathway and the core pathway of PLA synthesis. Furanone effectively inhibited the production of L. plantarum L3 PLA. In addition, Western blot analysis demonstrated that luxS, araT, and ldh were the key proteins regulating PLA production. This study reveals the regulatory mechanism of PLA based on the LuxS/AI-2 QS system, which provides a theoretical basis for the efficient and large-scale production of PLA in industries in the future.

Keywords: Aromatic aminotransferase; Furanone; Lactate dehydrogenase; Phenyllactic acid; S-ribosomal homocysteine lyase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins* / metabolism
Lactates / metabolism
Polyesters
Quorum Sensing*

Substances

3-phenyllactic acid
Bacterial Proteins
Lactates
Polyesters