Primary structure of the glycans of porcine pancreatic lipase

B Fournet; Y Leroy; J Montreuil; J Dĕcaro; M Rovery; J A van Kuik; J F Vliegenthart

doi:10.1111/j.1432-1033.1987.tb13709.x

Primary structure of the glycans of porcine pancreatic lipase

Eur J Biochem. 1987 Dec 30;170(1-2):369-71. doi: 10.1111/j.1432-1033.1987.tb13709.x.

Authors

B Fournet¹, Y Leroy, J Montreuil, J Dĕcaro, M Rovery, J A van Kuik, J F Vliegenthart

Affiliation

¹ Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq., France.

PMID: 3691527
DOI: 10.1111/j.1432-1033.1987.tb13709.x

Abstract

The glycan primary structure of the main glycopeptide fraction obtained by pronase and carboxypeptidase A digestions of porcine pancreatic lipase has been investigated by 500-MHz 1H-NMR spectroscopy and methylation analysis. The results demonstrate that the glycopeptide fraction was a mixture containing the following structures: (formula; see text)

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carbohydrate Conformation
Carbohydrate Sequence
Glycoproteins* / isolation & purification
Lipase* / isolation & purification
Molecular Sequence Data
Pancreas / enzymology*
Swine

Substances

Glycoproteins
Lipase