Abstract
The VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of host cell and viral mRNAs late after infection. The growth of a viral mutant that is unable to produce the RNA is inhibited by interferon, while wild-type virus is not affected. VAI RNA prevents activation of the interferon-induced P1/eIF-2 alpha kinase. This inhibition can be reproduced in extracts of interferon-treated cells where purified VAI RNA prevents activation of latent kinase by double-stranded RNA.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviruses, Human / drug effects
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Adenoviruses, Human / genetics
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Adenoviruses, Human / physiology*
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Cell Line
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Embryo, Mammalian
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Enzyme Activation / drug effects
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Humans
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Interferon Type I / pharmacology*
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Kidney
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Phosphorylation
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Protein Biosynthesis
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Protein Kinase Inhibitors*
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RNA, Double-Stranded / pharmacology
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RNA, Small Nuclear / physiology*
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RNA, Viral / physiology*
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eIF-2 Kinase
Substances
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Interferon Type I
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Protein Kinase Inhibitors
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RNA, Double-Stranded
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RNA, Small Nuclear
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RNA, Viral
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eIF-2 Kinase