In-situ growth of covalent organic framework on stainless steel needles as solid-phase microextraction probe coupled with electrospray ionization mass spectrometry for rapid and sensitive determination of tricyclic antidepressants in biosamples

Jiahao Yuan; Weini Huang; Wei Tong; Zihan Chen; Heming Li; Jiajing Chen; Zian Lin

doi:10.1016/j.chroma.2023.463955

In-situ growth of covalent organic framework on stainless steel needles as solid-phase microextraction probe coupled with electrospray ionization mass spectrometry for rapid and sensitive determination of tricyclic antidepressants in biosamples

J Chromatogr A. 2023 Apr 26:1695:463955. doi: 10.1016/j.chroma.2023.463955. Epub 2023 Mar 28.

Authors

Jiahao Yuan¹, Weini Huang¹, Wei Tong¹, Zihan Chen¹, Heming Li¹, Jiajing Chen¹, Zian Lin²

Affiliations

¹ Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
² Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address: zianlin@fzu.edu.cn.

PMID: 37004299
DOI: 10.1016/j.chroma.2023.463955

Abstract

Tricyclic antidepressants (TCAs) including amitriptyline (AT), doxepin (DOX) and nortriptyline (NT) are the first-line drugs for the clinical treatment of depression; however, monitoring TCA concentrations in biological fluids and tissues is necessary to improve therapeutic effect and determine the cause of death in patients. It is of great significance to develop a rapid and sensitive method for real-time monitoring of TCAs in various biosamples. In this work, we fabricated a novel covalent organic framework (COF) based solid-phase microextraction (SPME) probe by an in-situ step-by-step strategy, which was obtained by sequentially modifying 1,3,5-tri (4-aminophenyl) benzene (TPB) and 2, 5-divinylbenzaldehyde (DVA) on the surface of polydopamine layer. The TPB-DVA-COF-SPME probe possessed high specific surface area (1244 m²·g ^- ¹), regular pores (3.23 nm), good hydrophobicity and stability, resulting in efficient enrichment for TCAs. Furthermore, the combination of TPB-DVA-COF-SPME probe and ambient electrospray ionization mass spectrometry system (ESI/MS) was firstly proposed for rapid and sensitive determination of TCAs in biosamples. As a result, the developed method exhibited low limits of detection (LODs) (0.1-0.5 μg∙L ^- ¹), high enrichment factors (39-218), and low relative standard deviations (RSDs) for one probe (1.2-3.8%) and probe-to-probe (2.0-3.7%). Benefiting from these outstanding performance, TPB-DVA-COF-SPME probe was further successfully applied to biosamples (i.e., serum, liver, kidney, and brain) with excellent reusability, indicating the promising applicability of the TPB-DVA-COF-SPME-ESI/MS as a powerful tool for drug monitoring.

Keywords: Covalent organic framework; Electrospray ionization mass spectrometry; Solid-phase microextraction; Tricyclic antidepressants.

MeSH terms

Antidepressive Agents, Tricyclic* / analysis
Humans
Metal-Organic Frameworks*
Needles
Solid Phase Microextraction / methods
Spectrometry, Mass, Electrospray Ionization / methods
Stainless Steel / chemistry

Substances

Antidepressive Agents, Tricyclic
Metal-Organic Frameworks
Stainless Steel