A cloth-based single-working-electrode electrochemiluminescence sensor for simultaneous detection of diabetes complication markers

Wei Lai; Yi Liang; Xinyuan Mao; Kaifa Xue; Chunsun Zhang

doi:10.1016/j.aca.2023.341121

A cloth-based single-working-electrode electrochemiluminescence sensor for simultaneous detection of diabetes complication markers

Anal Chim Acta. 2023 May 8:1254:341121. doi: 10.1016/j.aca.2023.341121. Epub 2023 Mar 20.

Authors

Wei Lai¹, Yi Liang¹, Xinyuan Mao¹, Kaifa Xue¹, Chunsun Zhang²

Affiliations

¹ MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
² MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China. Electronic address: zhangcs@scnu.edu.cn.

PMID: 37005028
DOI: 10.1016/j.aca.2023.341121

Abstract

As one of the most common noninfectious diseases, diabetes and diabetic complications (DDC) have attracted great attention in the field of life and health. However, simultaneous detection of DDC markers usually requires labor- and time-consuming steps. Here, a novel cloth-based single-working-electrode electrochemiluminescence (SWE-ECL) sensor was designed for the simultaneous detection of multiple DDC markers. For this sensor, three independent ECL cells are distributed on the SWE, which is a simplification of the configuration of traditional sensors for simultaneous detection. In this way, the modification processes and ECL reactions occur at the back of the SWE, eliminating the adverse effects caused by human intervention on the electrode. Under optimized conditions, glucose, uric acid and lactate were determined, with corresponding linear dynamic ranges of 80-4000 μM, 45-1200 μM and 60-2000 μM, and detection limits of 54.79 μM, 23.95 μM and 25.82 μM, respectively. In addition, the cloth-based SWE-ECL sensor exhibited good specificity and satisfactory reproducibility, and its actual application potential was verified by measuring complex human serum samples. Overall, this work developed a simple, sensitive, low-cost and rapid method for the simultaneous quantitative determination of multiple markers related to DDC and demonstrated a new route for multiple-marker detection.

Keywords: Cloth-based sensor; Diabetes and diabetic complications; Electrochemiluminescence; Simultaneous detection; Single-working-electrode.

MeSH terms

Biosensing Techniques* / methods
Diabetes Mellitus* / diagnosis
Electrochemical Techniques / methods
Electrodes
Humans
Limit of Detection
Luminescent Measurements / methods
Photometry
Reproducibility of Results